高氧暴露对早产大鼠肺组织中HO-1与iNOS表达的影响

全裕凤,郑明慈,张华,张培林,张红

中国当代儿科杂志 ›› 2011, Vol. 13 ›› Issue (7) : 577-580.

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中国当代儿科杂志 ›› 2011, Vol. 13 ›› Issue (7) : 577-580.
论著·实验研究

高氧暴露对早产大鼠肺组织中HO-1与iNOS表达的影响

  • 全裕凤,郑明慈,张华,张培林,张红
作者信息 +

Expression of heme oxygenase-1 and inducible nitric oxide synthase in the lungs of hyperoxia-exposed preterm rats

  • QUAN Yu-Feng, ZHENG Ming-Ci, ZHANG Hua, ZHANG Pei-Lin, ZHANG Hong
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文章历史 +

摘要

目的:探讨血红素加氧酶-1(HO-1)与诱导型一氧化氮合酶(iNOS)在高氧肺损伤大鼠中的表达及作用。方法:将3日龄早产Sprague-Dawley大鼠64只随机分为高氧组、空气组(每组32只),于实验 3 d及 7 d时,分别检测空气组和高氧组肺组织 HO-1活性、肺泡灌洗液中 NO、肺组织病理学改变及 HO-1、iNOS 在肺内的分布和表达(免疫组织化学方法)。结果:3 d、7 d高氧组存在明显急性肺炎症性改变,iNOS 在中性粒细胞的表达、灌洗液中 NO 含量明显高于空气组 (P均<0.01),且7 d高氧组高于3 d高氧组(P<0.05);3 d、7 d 时高氧组巨噬细胞 HO-1 表达高于空气组(分别P<0.05,P<0.01),且7 d高氧组显著高于3 d高氧组(P<0.01) 。结论:HO-1 与 iNOS在高氧肺损伤大鼠中的表达是增高的,HO-1 与 iNOS 均可能参与了高氧肺损伤。

Abstract

OBJECTIVE: To study the expression and the role of heme oxygenase-1 (HO-1) and inducible nitric oxide synthase (iNOS) in preterm rats with hyperoxia-induced lung injuries. METHODS: Sixty-four three-day-old preterm Sprague-Dawley rats were randomly assigned to a hyperoxia group (90% oxygen exposure) and a control group (room air exposure), with 32 rats in each group. After 3 days or 7 days of exposure, the lung activity of HO-1 and nitric oxide (NO) contents in bronchoalveolar lavage fluid (BALF), pulmonary histopathologic changes, and the cellular distribution and expression of HO-1 and iNOS in the lungs were measured. RESULTS: After 3 days and 7 days of exposure, the hyperoxia group showed acute lung injuries characterized by the presence of hyperaemia, red cell extravasation and inflammatory infiltration. The NO contents in BALF and the iNOS expression in the lungs increased significantly in the hyperoxia group compared with those in the control group 3 and 7 days after exposure. The expression of HO-1 in macrophages in the lungs increased significantly in the hyperoxia group compared with that in the control group 3 and 7 days after exposure. The NO contents in BALF and the iNOS and HO-1 expression in the lungs increased significantly 7 days after hyperoxia exposure compared with 3 days after hyperoxia exposure. CONCLUSIONS: iNOS and HO-1 levels in the lungs increase in preterm rats with hyperoxia-induced lung injuries, suggesting that iNOS and HO-1 may play roles in hyperoxia-induced pulmonary injuries.

关键词

血红素加氧酶 / 诱导型一氧化氮合酶 / 高氧 / / 早产大鼠

Key words

Heme oxygenase-1 / Inducible nitric oxide synthase / Hyperoxia / Lung / Preterm rats

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全裕凤,郑明慈,张华,张培林,张红. 高氧暴露对早产大鼠肺组织中HO-1与iNOS表达的影响[J]. 中国当代儿科杂志. 2011, 13(7): 577-580
QUAN Yu-Feng, ZHENG Ming-Ci, ZHANG Hua, ZHANG Pei-Lin, ZHANG Hong. Expression of heme oxygenase-1 and inducible nitric oxide synthase in the lungs of hyperoxia-exposed preterm rats[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(7): 577-580
中图分类号: R-33   

参考文献

[1]Farkas I, Maróti Z, Katona M, Endreffy E, Monostori P, Máder K, et al. Increased heme oxygenase-1 expression in premature infants with respiratory distress syndrome [J]. Eur J Pediatr, 2008, 167(12):1379-1383.

[2]Dennery PA, Rodgers PA, Lum MA, Jennings BC, Shokoohi V. Hyperoxic regulation of lung heme oxygenase in neonatal rats [J]. Pediatr Res, 1996, 40(6): 815-821.

[3]Ito Y, Betsuyaku T, Moriyama C, Nasuhara Y, Nishimura M. Aging affects lipopolysaccharide-induced upregulation of heme oxygenase-1 in the lungs and alveolar macrophages[J]. Biogerontology, 2009, 10(2): 173-180 .

[4]Pascal T, Debacq-Chainiaux F, Boilan E, Ninane N, Raes M, Toussaint O. Heme oxygenase-1 and interleukin-11 are overexpressed in stress-induced premature senescence of human WI-38 fibroblasts induced by tert-butylhydroperoxide and ethanol[J]. Biogerontology, 2007, 8(4): 409-422.

[5]全裕凤,郑明慈,张华,邓毅,张红.高体积分数氧暴露早产大鼠肺组织血红素加氧酶-1的表达及活性变化 [J] . 实用儿科临床杂志,2009,4(6):428-430.

[6]全裕凤,郑明慈,张华,张培林,张红.血红素氧合酶-1抑制剂锌原卟啉对早产鼠高氧肺损伤的影响 [J] . 实用儿科临床杂志,2009,24(18): 1409-1411.

[7]戎小平,邵勤,郭秀霞,江莲. 足月小于胎龄儿脐血中血红素氧合酶-1的测定及其在脐血管上的表达 [J]. 中国实用儿科杂志,2008,23(1):21-24.

[8]Chen W, Jia Z, Zhu H, Li Y, Misra HP. Ethyl pyruvate inhibits peroxynitrite-induced DNA damage and hydroxyl radical generation: implications for neuroprotection[J]. Neurochem Res, 2010, 35(2): 336-342.

[9]张向峰,梁瑛.高氧所致小鼠急性肺损伤时一氧化氮合成酶的表达(英文) [J]. 中华急诊医学杂志,2004,13(6):365-367.

[10]Auten RL, Mason SN, Whorton MH, Lampe WR, Foster WM, Goldberg RN, et al. Inhaled  ethyl nitrite prevents hyperoxia-impaired  postnatal alveolar development in newborn rats [J]. Am J Res Crti Car Med, 2007, 176(3): 291-299.

[11]Suttner DM, Sridhar K, Lee CS. Protective effects of transient HO-1 overexpressionon susceptibility to oxygen toxicity in lung cells[J]. Am J Physiol, 1999, 276(3 Pt 1): 443-448.

[12]Suttner DM, Dennery PA. Reversal of HO-1 related cytoprotection with increased expression is due to reactive iron[J]. FASEB J ,1999,13(13):1800-1805.

[13]张帆,夏中元,欧阳静萍.肠缺血再灌注致肺损伤时肺内HO-1/CO与 iNOS/NO 相互作用的研究[J].中国急救医学,2005,25(10) :728-730.

[14]Yang NC, Lu LH, Kao YH, Chau LY. Heme oxygenase-1 attenuates interleukin-1β-induced nitric oxide synthase expression in vascular smooth muscle cells [J].Biomed Sci, 2004, 11(6): 799-809.

[15]吕勇,孙波.吸入一氧化氮对儿科患者高铁血红蛋白浓度的影响[J].中国当代儿科杂志,2008,10(2):257-258.

[16]Siow RC, Sato H, Mann GE. Heme oxygenase-carbon monoxide signaling pathway in atherosclerosis: anti-atherogenic actions of bilirubin and carbon monoxide[J].Cardiovasc Res, 1999, 41(2): 385-390.

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