目的：探讨早期丰富环境对大鼠行为发育及血清皮质酮的影响。方法：将45只新生大鼠随机分为丰富环境组、空白对照组和隔离环境组，于大鼠生后31 d分别采用开场实验、Lat迷宫测验评价大鼠焦虑、烦躁等行为；采用放射免疫学方法测定大鼠血清皮质酮水平。结果：丰富环境组血清皮质酮水平（8±3 ng/mL）较空白对照组（11±4 ng/mL）及隔离环境组（22±4 ng/mL）明显降低,差异有统计学意义（P<0.01）。开场实验结果显示，丰富环境组大鼠穿越格子数、直立次数、理毛次数明显少于空白对照组及隔离环境组大鼠（P<0.05）。Lat迷宫试验结果显示，丰富环境组大鼠穿越角落数、直立次数、斜搭在墙上的频率明显少于空白对照组与隔离环境组大鼠（P<0.05）。结论：早期丰富环境使大鼠能保持较低的皮质酮水平，减缓焦虑、烦躁等行为，对促进脑发育有重要意义。
Abstract
OBJECTIVE: To study the effects of early enriched environment on behavioral development and serum corticosterone level in rats. METHODS: Forty-five neonatal rats were randomly assigned into three groups：blank control, enriched environment and isolated environment. The open-field environment test and the Lat maze test were performed to assess anxiety/irritability-related behaviors of the rats on postnatal day 31. The level of serum corticosterone was measured by radioimmunology assay. RESULTS: The level of serum corticosterone in the enriched environment group (8±3 ng/mL) was significantly lower than the blank control (11±4 ng/mL) and the isolated groups (22±4 ng/mL) (P<0.01). The open-field environment test showed that the numbers of passing panels, keeping an erect posture and grooming were less than those in the blank control and the isolated groups (P<0.05). According to the results of the Lat maze test, the frequencies of running across the corner, keeping an erect posture and leaning against the wall in the enriched environment group were less than those in the blank control and the isolated groups (P<0.05). CONCLUSIONS: Early enriched environment can decrease serum corticosterone level and thus alleviates anxiety and irritability in rats. It may play an important role in the improvement of brain development.
关键词
早期环境 /
皮质酮 /
行为发育 /
大鼠
Key words
Early environment /
Corticosterone /
Behavior development /
Rats
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