Abstract:OBJECTIVE: To study the effects of huai qi huang, a traditional Chinese medicine, on cytokines Th1, Th2 and Th17 levels and alveolar macrophage phagocytosis in asthmatic rats sensitized by ovalbumin (OVA). METHODS: Forty male Sprague-Dawley rats were randomly divided into five groups: normal control, untreated asthma, budesonide-treated, huai qi huang-treated and budesonide+huai qi huang-treated asthma (n=8 each). Asthma was induced by OVA sensitization and challenge. The levels of IL-4, IFN-γ and IL-17 in plasma and bronchoalveolar lavage fluid (BALF) were measured using ELISA. The phagocytosis of alveolar macrophages which were isolated and purified from BALF was evaluated by the colorimetric assay. RESULTS: The levels of IL-4 and IL-17 increased, in contrast, the IFN-γ level decreased in plasma and BALF in the untreated asthma group compared with those in the normal control group. The IFN-γ level in the huai qi huang-treated asthma group was higher than that in the untreated asthma group. The IFN-γ level increased and the IL-17 level decreased more significantly in the budesonide+huai qi huang-treated asthma group when compared with the budesonide and huai qi huang alone treatment groups. The phagocytosis of alveolar macrophages in the untreated asthma group was lower than that in the normal control group. Huai qi huang alone or combined with budesonide increased the phagocytosis of alveolar macrophages compared with the normal control, untreated asthma and budesonid-treated asthma groups. The levels of IFN-γ in plasma and BALF were positively correlated with the phagocytosis of alveolar macrophages. CONCLUSIONS: The levels of IL-4 and IL-17 increase and the IFN-γ level decreases in plasma and BALF, and the phagocytosis of alveolar macrophages decreases in asthmatic rats. Huai qi huang treatment may increase the IFN-γ expression in plasma and BALF and the phagocytosis of alveolar macrophages in asthmatic rats. There is a synergistic effect between huai qi huang and glucocorticoids.
LI Hong-Mei,HE Qing-Nan,LI Xiao-Yan et al. Effects of huai qi huang on cytokines Th1, Th2 and Th17 and phagocytosis of alveolar macrophages in rats with asthma[J]. CJCP, 2011, 13(9): 747-750.
[1]Molet S, Hamid Q, Davoine F, Nutku E, Taha R, Pagé N, et al. IL-17 is increased in asthmatic airways and induces human bronchial fibroblasts to produce cytokines[J].J A1lergy Clin Immunol, 2001, 108(3): 430-438.
[2]Sirois J, Bissonnette EY. Alveolar macrophages of allergic resistant and susceptible strains of rats show distinct cytokine profiles[J].Clin Exp Immunol, 2001, 126(1): 9-15.
[3]Palmans E, Kips JC, Pauwels PA. Prolonged allergen exposure induces structural airway changes in sensitized rats[J]. Am J Respir Crit Care Med, 2000, 161(2): 627-635.
[5]Yang Y, Wanshun L, Baogin H, Changhong W, Chenwei F, Bing L, et al. The antioxidative and immunostimulating properties of D-glucosamine[J].Int Immunopharmacol, 2007, 7(1): 29-35.
[6]Harrington LE, Mangan PR, Weaver CT. Expanding the effector CD4 T-cell repertoire: the Th17 lineage[J]. Curr Opin Immunol, 2006, 18(3): 349-356.
[7]Dong C. TH17 cells in development:an updated view of their molecular identity and genetic programming[J].Nat Rev Immunol, 2008, 8(5): 337-348.
[8]Bullens DM, Truyen E, Coteur L, Dilissen E, Hellings PW, Dupont LJ, et al. IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?[J].Respir Res, 2006, 7: 135.
[10]Alexis NE, Soukup J, Nierkens S, Becker S. Association between airway hyperreactivity and bronchial macrophage dysfunction in individuals with mild asthma[J]. Am J Physiol Lung Cell Mol Physiol, 2001, 280(2): L369-L375.