Abstract：Objective To study the changes of endogenous leukemia inhibitory factor（LIF) in neonatal rats with periventricular leukomalacia（PVL). Methods A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia（HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP. Results At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group（PPConclusion LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.
FAN Yu-Ying,YU Tao,ZHANG Jun-Mei et al. Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia[J]. CJCP, 2014, 16(9): 933-938.
Huang Z, Liu J, Cheung PY, et al.Long-term cognitive impairment and myelination deficiency in a rat model of perinatal hypoxic-ischemic brain injury [J].Brain Research,2009, 1301:100-109.
[2]
Buser JR, Maire J, Riddle A, et al.Arrested preoligodendrocyte maturation contributes to myelination failure in premature infants [J].Annals of Neurology, 2012, 71(1): 93-109.
[3]
Bain JM, Ziegler A, Yang Z, et al.TGFβ1 stimulates the over-production of white matter astrocytes from precursors of the "brain marrow" in a rodent model of neonatal encephalopathy[J].PloS One, 2010, 5(3): e9567.
[4]
Butzkueven H, Emery B, Cipriani T, et al.Endogenous leukemia inhibitory factor production limits autoimmune demyelination and oligodendrocyte loss [J].Glia, 2006, 53(7): 696-703.
[5]
Leibinger M, Müller A, Andreadaki A, et al.Neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and leukemia inhibitory factor [J].J Neurosci, 2009, 29(45): 14334-14341.
[6]
Yu-Ying F, Jun-Mei Z, Wang H, et al.Leukemia inhibitory factor inhibits the proliferation of primary rat astrocytes induced by oxygen-glucose deprivation [J].Acta Neurobiol Exp（Wars),2013, 73(4): 485-494.
[7]
Covey MV, Levison SW.Leukemia inhibitory factor participates in the expansion of neural stem/progenitors after perinatal hypoxia/ischemia [J].Neuroscience, 2007, 148(2): 501-509.
[8]
Slevin M, Krupinski J, Mitsios N, et al.Leukemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke [J].Eur J Neurol, 2008, 15(1):29-37.
[9]
Soilu-Hänninen M, Broberg E, Röyttä M, et al.Expression of LIF and LIF receptor beta in Alzheimer's and Parkinson's diseases [J].Acta Neurol Scand, 2010, 121(1): 44-50.
Lapp DW, Zhang SS, Barnstable CJ.Stat3 mediates LIF-induced protection of astrocytes against toxic ROS by upregulating the UPC2 Mrna pool [J].Glia, 2014, 62(2): 159-170.
[14]
Asano H, Aonuma M, Sanosaka T, et al.Astrocyte differentiation of neural precursor cells is enhanced by retinoic acid through a change in epigenetic modification [J].Stem Cells, 2009, 27(11):2744-2752.
[15]
Liu J, Zang D.Response of neural precursor cells in the brain of Parkinson's disease mouse model after LIF administration [J].Neurol Res, 2009, 31(7): 681-686.
[16]
Fischer R1, Wajant H, Kontermann R, et al.Astrocyte-specific activation of TNFR2 promotes oligodendrocyte maturation by secretion of leukemia inhibitory factor [J].Glia, 2014, 62(2):272-283.
