肺炎支原体肺炎患儿血清TNF-&alpha;、IL-6及半乳凝素3浓度测定的临床意义

田芳; 韩波; 段梅

doi:10.7499/j.issn.1008-8830.2014.10.009

PDF(1190 KB)

HTML

中国当代儿科杂志 ›› 2014, Vol. 16 ›› Issue (10) : 1001-1004. DOI: 10.7499/j.issn.1008-8830.2014.10.009

世界肺炎日专题

肺炎支原体肺炎患儿血清TNF-α、IL-6及半乳凝素3浓度测定的临床意义

田芳¹, 韩波², 段梅³

作者信息 +

Serum tumor necrosis factor-α, interleukin -6 and galctin-3 concentrations in children with Mycoplasma pneumoniae pneumonia

TIAN Fang¹, HAN Bo², DUAN Mei³

Author information +

文章历史 +

摘要

目的检测肺炎支原体肺炎（Mycoplasma pneumoniae pneumonia， MPP）患儿血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和半乳凝素3（Gal-3）浓度的变化，探讨其在MPP 中的作用。方法应用ELISA 双抗体夹心法对48 例MPP 急性期患儿（重症组和轻症组分别为21 例和27 例）和30 例健康儿童（对照组）血清中TNF-α、IL-6、Gal-3 浓度进行检测。结果治疗前重症组和轻症组患儿血清中TNF-α、IL-6、Gal-3 浓度明显高于对照组（P<0.05），且重症组TNF-α、IL-6 和Gal-3 水平明显高于轻症组（P<0.05）。两组患儿治疗后其浓度均明显下降（P<0.05）。结论血清 TNF-α、IL-6 和Gal-3 在MPP 的发生发展过程中起重要作用，其血清浓度可反映MPP 患儿的病情轻重。

Abstract

Objective To study the changes in serum tumor necrosis factor-α (TNF- α), interleukin-6 (IL-6) and galctin-3 (Gal-3) concentrations in children with Mycoplasma pneumoniae pneumonia (MPP), and their roles in MPP. Methods Serum TNF-α, IL-6 and Gal-3 concentrations were measured using double antibody sandwich ELISA in 48 children with acute MPP (severe: 21 cases; mild: 27 cases) and in 30 healthy children (control group). Results Serum concentrations of TNF-α, IL-6 and Gal-3 in both the severe and mild groups were significantly higher than in the control group before treatment. The concentrations of the three indexes in the severe group were significantly higher than in the mild group. Serum concentrations of the three indexes decreased significantly after treatment in both groups. Conclusions Serum TNF-α, IL-6 and Gal-3 play important roles in the occurrence and development of pediatric MPP and their levels are associated the severity of this disorder.

导出引用

田芳, 韩波, 段梅. 肺炎支原体肺炎患儿血清TNF-α、IL-6及半乳凝素3浓度测定的临床意义[J]. 中国当代儿科杂志. 2014, 16(10): 1001-1004 https://doi.org/10.7499/j.issn.1008-8830.2014.10.009

TIAN Fang, HAN Bo, DUAN Mei. Serum tumor necrosis factor-α, interleukin -6 and galctin-3 concentrations in children with Mycoplasma pneumoniae pneumonia[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(10): 1001-1004 https://doi.org/10.7499/j.issn.1008-8830.2014.10.009

参考文献

[1] 朱影, 刘晓琳, 叶玉兰. 肺炎支原体所致大叶性肺炎46 例临床分析[J]. 中华全科医学, 2012, 10(4): 446-447.
[2] 崔娟, 王佳, 姚慧生, 等. 2006-2010 年儿童肺炎支原体感染流行病学分析[J]. 中国实用儿科杂志, 2013, 28(6): 560-561.
[3] 岳保珠, 吴春莲, 刘丽平. 儿童肺炎支原体感染性大叶性肺炎l0 年发病研究[J]. 医学综述, 2010, 16(13): 2071-2073.
[4] 张永明, 刘秀云, 江载芳. 儿童肺炎支原体肺炎合并肺不张发病率及预后研究[J]. 中国实用儿科杂志, 2010, 25(2): 143-146.
[5] 徐晓群, 周波, 吴新萍. 肺炎患儿血清白细胞介素-10、可溶性细胞介素-2 受体水平的临床意义[J]. 实用儿科临床杂志,2004, 19(2): 124.
[6] 余峰, 崔敏娴, 姚冬莉. 儿童重症肺炎血清致炎性细胞因子变化及意义探讨[J]. 医学研究杂志, 2007, 36(2): 74-76.
[7] 李晓芹. 重症支原体肺炎患儿血清TNF-α、IL-6、IL-8 检测及其临床意义[J]. 实用诊断与临床治疗杂志, 2005, 19(9):649-650.
[8] Narita M. Mycoplasma pneumoniae encephalitis andcytokines[J]. Pediatrics, 2008, 121(1): 224-225.
[9] 陆权. 儿童社区获得性肺炎管理指南（2013 修订） [J]. 中华儿科杂志, 2013, 51(10): 745-752.
[10] Harris M, Clerk J, Coote N, et al. British Thoracic Societyguidelines for the managerment of community acquiredpneumonia in children: update 2011[J]. Thorax, 2011, 66 (Suppl2): ii1-23.
[11] Zar HJ, Jeena P, Argent A, et al; Working Groups of thePaediatric Assembly of the South African Thoracic Society.Diagnosis and management of community-acquired pneumoniain childhood-South African Thoracic Society Guidelines[J]. SAfr Med J, 2005, 95(12 Pt 2): 977-981.
[12] Gove S. Integrated management of childhood illness byoutpatient health workers: technical basis and overview. TheWHO Working Group on Guidelines for Integrated Managementof the Sick Child[J]. Bull World Health Organ, 1997,75(Suppl 1): 7-24.
[13] Bradley JS1, Byington CL, Shah SS, et al. The managementof community-acquired pneumonia in infants and childrenolder than 3 months of age: clinical practice guidelines by thePediatric Infectious Diseases Society and the Infectious DiseasesSociety of America[J]. Clin Infect Dis, 2011, 53(7): e25-e76.
[14] Ahn KK, Kwon D, Jung K, et al. Identification of interleukin-1,tumor necrosis factor-alpha, and interleukin-6 expressionin lungs from pigs naturally infected with Mycoplasmahyopneumoniae by in situ hybridization[J]. J Vet Med Sci, 2009,71(4): 441-445.
[15] Mukhopadhyay S, Hoidal JR, Mukherjee TK. Role of TNFalphain pulmonary pathophysiology[J]. Respir Res, 2006,7(125): 1-9.
[16] Baluk P, Yao LC, Feng J, et al. TNF-α drives remodeling ofblood vessels and lymphatics in sustained airway inflammationin mice[J]. Clin Invest, 2009, 119(10): 2954-2964.
[17] 俞敏, 田兆方. 急性肺损伤时损伤标志物的研究进展观[J].中国当代儿科杂志, 2013, 15(10): 94-98.
[18] 曹岩, 刘红, 卢晟晔, 等. 儿童肺炎支原体肺炎血清中TNF-α、IL-6、IL-8 和IL-10 浓度测定及其临床意义的研究[J].中国妇幼保健, 2008, 23(18): 2575-2578.
[19] 郦银芳, 于莹, 陆志芳. IL-13 和TNF-α 在肺炎支原体肺炎儿童血清中的表达及意义[J]. 中国当代儿科杂志, 2010,12(4): 275-277.
[20] 庞焕香, 乔红梅, 成焕吉, 等. 支原体肺炎患儿肺泡灌洗液中TNF-α、IL-6、IL-10 水平检测及意义[J]. 中国当代儿科杂志, 2011, 13(10): 808-810.
[21] 叶新明，钱克俭. 重症肺炎支原体肺炎肺泡灌洗液中TNF-α、IL-6、IL-8、IL-10 水平观察[J]. 南昌大学报( 医学版),2010, 50(4): 72-74.
[22] Antunes G, Evans SA, Lordan JL. Systemic eytokine levelsin community-acquired pneumonia and their association withdisease severity[J]. Eur Respir, 2002, 20(4): 990-995.
[23] Ben-Rafael Z, Orvieto R. Cytokines involvement in reproduction[J]. Fertile Steril, 1992, 58(6): 1093-1099.