不同胎龄新生儿细胞和体液免疫功能检测

李燕; 韦秋芬; 潘新年; 蒙丹华; 刘先知; 许靖; 韦玮

doi:10.7499/j.issn.1008-8830.2014.11.009

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中国当代儿科杂志 ›› 2014, Vol. 16 ›› Issue (11) : 1118-1121. DOI: 10.7499/j.issn.1008-8830.2014.11.009

论著·临床研究

不同胎龄新生儿细胞和体液免疫功能检测

李燕, 韦秋芬, 潘新年, 蒙丹华, 刘先知, 许靖, 韦玮

作者信息 +

Cellular and humoral immunity in preterm infants of different gestational ages

LI Yan, WEI Qiu-Fen, PAN Xin-Nian, MENG Dan-Hua, LIU Xian-Zhi, XU Jing, WEI Wei

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摘要

目的了解不同胎龄新生儿的免疫功能特点.方法 2012 年6 月1 日至2013 年6 月1 日收治的115 例无感染早产儿,根据出生时胎龄分为早期早产儿组(28~33⁺⁶ 周,n=57)和晚期早产儿组(34~36⁺⁶ 周,n=58);同期选取88 例健康足月儿(37~41⁺⁶ 周)为对照.于出生后24 h 内采集各组静脉血,采用流式细胞仪检测各组淋巴细胞亚群CD3⁺、CD4⁺、CD8⁺、CD19⁺ 和NK 细胞,并根据血常规结果计算各淋巴细胞亚群含量;采用免疫比浊法检测各组血清IgG、IgA 和IgM 含量.结果早期和晚期早产儿组CD3⁺、CD4⁺ 百分比及CD4⁺/CD8⁺ 比值均高于足月儿组(P<0.05),而CD8⁺、CD19⁺ 和NK 细胞百分比均低于足月儿组(均P<0.05);早期和晚期早产儿组总淋巴细胞、CD3⁺、CD4⁺、CD8⁺、CD19⁺ 和NK 细胞含量均低于足月儿组(P<0.05),且上述指标在晚期早产儿组的水平均高于早期早产儿组(P<0.05);早期和晚期早产儿组血清IgG 水平低于足月儿组(P<0.05),而IgA、IgM 含量在3 组间差异无统计学意义(P>0.05).结论新生儿胎龄影响其细胞免疫及体液免疫功能;随胎龄增长,新生儿免疫功能将逐渐完善.

Abstract

Objective To investigate the characteristics of immune function in newborn infants of different gestational ages. Methods A total of 115 premature infants free of infection between June 1, 2012 and June 1, 2013 were divided into two groups according to their gestational age at birth:early preterm infant group (28-33⁺⁶ weeks,n=57) and late preterm infant group (34-36⁺⁶ weeks, n=58). Meanwhile, 88 full-term infants (37-41⁺⁶ week) wererecruited to the control group. Venous blood samples were collected within 24 hours after birth. The percentages oflymphocyte subsets, such as CD3⁺, CD4⁺, CD8⁺, and CD19⁺ T cells and natural killer (NK) cells were measured by flow cytometry, and the absolute count of each population was calculated using the results from routine blood work.Concentrations of serum IgG, IgA, and IgM were measured by immunoturbidimetry. Results Both preterm infantgroups had significantly higher percentages of CD3⁺ and CD4⁺ T cells and CD4⁺/CD8⁺ ratio (P<0.05) and significantlylower percentages of CD8⁺ and CD19⁺ T cells and NK cells (P<0.05), as compared with the full-term infant group. Theabsolute counts of total lymphocytes, CD3⁺, CD4⁺, CD8⁺, and CD19⁺ T cells, and NK cells in both preterm infant groupswere significantly lower than those in the full-term infant group (P<0.05), and the above parameters in the late preterminfant group were significantly higher than those in the early preterm infant group (P<0.05). Both preterm infant groups showed significantly lower concentrations of serum IgG than the full-term infant group (P<0.05), while no significant differences in concentrations of serum IgA and IgM were observed between the three groups (P>0.05). Conclusions Neonatal gestational age has an effect on cellular and humoral immunity. The immune function gradually improves with increasing gestational age.

导出引用

李燕, 韦秋芬, 潘新年, 蒙丹华, 刘先知, 许靖, 韦玮. 不同胎龄新生儿细胞和体液免疫功能检测[J]. 中国当代儿科杂志. 2014, 16(11): 1118-1121 https://doi.org/10.7499/j.issn.1008-8830.2014.11.009

LI Yan, WEI Qiu-Fen, PAN Xin-Nian, MENG Dan-Hua, LIU Xian-Zhi, XU Jing, WEI Wei. Cellular and humoral immunity in preterm infants of different gestational ages[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(11): 1118-1121 https://doi.org/10.7499/j.issn.1008-8830.2014.11.009

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