Abstract：Objective To investigate the dynamic changes of intestinal 16S rDNA metagenome in healthy infants.Methods Seventeen fecal samples were collected at ages of 3 days, 1 month, 6 months and 1 year in 5 infants. Totalbacterial DNAs were extracted and submitted high throughout sequencing on the V6 viable region of 16S rDNA. Tagsand Operational Taxonomic Units (OTU) were then obtained and analysis of taxonomy, abundance and alpha diversitywere performed. Results In total 2 190.66 Mbp raw data in 17 samples were produced. The OTU numbers rangedfrom 36 to 308. The dominate phylum included Proteobacteria, Firmicutes and Bacteroidetes and Actinobacteria. The bacterial families >1% increased from only 2-4 per sample on day 3 to 7 at 1 or 6 months, 10 at 12 months. The averagenp Shannon and Simpson index on day 3, at 1 month, 6 months and 1 year were 1.117, 1.460, 2.088, 2.50 and 0.443, 0.408,0.229, 0.143 respectively. Conclusions Infants' intestines harbor abounding bacterial genomes. Distinct individual differences exist in infants in terms of intestinal bacterial abundance and composition. The abundance and diversity ofgut bacteria increase over time.
Matamoros S, Gras-Leguen C, Le Vacon F, et al. Development of intestinal microbiota in infants and its impact on health[J]. Trends Microbiol, 2013, 21(4):167-173.
[2]
Qin J, Li R, Raes J, et al. A human gut microbial gene catalogue established by metagenomic sequencing[J]. Nature, 2010, 464(7285):59-65.
[3]
Song S, Jarvie T, Hattori M. Our second genome-human metagenome:how next-generation sequencer changes our life through microbiology[J]. Adv Microb Physiol, 2013, 62:119-144.
[4]
Di Mauro A, Neu J, Riezzo G, et al. Gastrointestinal function development and microbiota[J]. Ital J Pediatr, 2013, 39:15.
[5]
Berrington JE, Stewart CJ, Embleton ND, et al. Gut microbiota in preterm infants:assessment and relevance to health and disease[J]. Arch Dis Child Fetal Neonatal Ed, 2013, 98(4):F286-F290.
[6]
Fan W, Huo G, Li X, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in infants during the six months of life[J]. J Microbiol Biotechnol, 2014, 24(2):133-143.
[7]
Yatsunenko T, Rey FE, Manary MJ, et al. Human gut microbiome viewed across age and geography[J]. Nature, 2012, 486(7402):222-227.
[8]
Fallani M, Young D, Scott J, et al. Intestinal microbiota of 6-week-old infants across Europe:geographic influence beyond delivery mode, breast-feeding, and antibiotics[J]. J Pediatr Gastroenterol Nutr, 2010, 51(1):77-84.
[9]
Biasucci G, Rubini M, Riboni S, et al. Mode of delivery affects the bacterial community in the newborn gut[J]. Early Hum Dev, 2010, 86(Suppl 1):13-15.
[10]
Arumugam M, Raes J, Pelletier E, et al. Enterotypes of the human gut microbiome [J]. Nature, 2011, 473(7346):174-180.
[11]
De Filippo C, Cavalieri D, Di Paola M, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa[J]. Proc Natl Acad Sci USA, 2010, 107(33):14691-14696.
