Abstract：Objective To study the alterations of follicular T helper cells (CD4+CXCR5+Tfh cells, Tfh) on circulating T lymphocytes in children with asthma, and to study the expression of transcription regulatory factors BCL-6 and BLIMP-1 mRNA. Methods Sixty-four children with asthma and 25 healthy controls were enrolled in this study. On the basis of the disease, the children with asthma were classified into acute phase group (n=36) and remission phase group (n=28). The flow cytometry was used to detect the proportion of CD4+CXCR5+Tfh cells on CD4+T lymphocytes. Real-time PCR was performed to detect the levels of BCL-6 mRNA and BLIMP-1 mRNA. The double-antibody Sandwich ELISA was used to detect plasma concentrations of total IgE, IL-2, IL-6 and IL-21. Results The proportion of CD4+CXCR5+Tfh cells was significantly higher in the acute group than in the control group and the remission group (PPPP+CXCR5+Tfh cells (r=0.76, r=0.46 respectively; Pr=-0.68, PConclusions The abnormal proportion of CD4+CXCR5+Tfh cells might be involved in the immunological pathogenesis of acute asthma in children. The increased expression of BCL-6 mRNA and decreased expression of BLIMP-1 mRNA as well as the alterations of plasma total IgE, cytokines IL-2, IL-6 and IL-21 in microenvironment might be account for the increased proportion of CD4+CXCR5+Tfh cells in children with acute asthma.
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