中国当代儿科杂志
  中文版
  English Version
  ISSN 2096-9228(online)
ISSN 1008-8830(print)
CN 43-1301/R
 
中国当代儿科杂志  2015, Vol. 17 Issue (1): 81-85    DOI:10.7499/j.issn.1008-8830.2015.01.018
论著·实验研究 最新目录| 下期目录| 过刊浏览| 高级检索 |
糖皮质激素对高氧诱导新生大鼠肺组织RAGE-NF-κB通路的影响
胡剑, 俞敏, 唐云, 田兆方
南京医科大学附属淮安第一医院新生儿科, 江苏 淮安 223300
Effects of glucocorticoid on RAGE-NF-κB pathway in hyperoxia-induced lung tissues of neonatal rats
HU Jian, YU Min, TANG Yun, TIAN Zhao-Fang
Department of Neonatology, Huai'an First Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, China
全文: PDF (1775 KB)   HTML(43)
输出: BibTeX | EndNote (RIS)      背景资料
摘要 目的 探讨RAGE-NF-κB 信号通路在新生大鼠高氧肺损伤过程中的变化,以及糖皮质激素对其的影响.方法 24 只新生大鼠随机分为空气对照组、高氧模型组和激素干预组.模型组新生大鼠于生后即暴露于95% 氧气浓度环境中1 周,空气对照组大鼠生后仅暴露于同室内空气中饲养7 d,激素干预组于造模后行尾静脉注射地塞米松(1 mg/kg),隔天1 次,共3 次.日龄13 d 时处死全部大鼠,RT-PCR 检测各组肺组织RAGEmRNA 及NF-κB mRNA的表达;Western blot检测RAGE 及NF-κB 蛋白水平;ELISA 法检测血清及肺泡灌洗液(BALF)中TNF-α 和sRAGE 含量;苏木精- 伊红染色后行肺组织病理学评估.结果 与对照组和激素干预组相比,高氧模型组肺组织RAGE、NF-κB 的mRNA 和蛋白表达水平均明显增高(均P<0.05),血清中sRAGE 含量明显升高(P<0.01),BALF 中sRAGE 含量下降(P<0.01);与对照组相比,激素干预组肺组织RAGE、NF-κB 的mRNA和蛋白表达水平明显升高(P<0.05),血清中sRAGE 含量明显升高(P<0.05),而BALF 中sRAGE 含量明显下降(P<0.05).结论 RAGE-NF-κB 通路在高氧诱导的新生大鼠肺损伤中活化增强,糖皮质激素可能通过下调RAGE-NF-κB 信号通路对高氧肺损伤发挥保护作用.
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
胡剑
俞敏
唐云
田兆方
关键词 糖皮质激素晚期糖基化终末产物受体肺损伤新生大鼠    
Abstract:Objective To explore the change of RAGE-NF-κB signaling pathway during the course of hyperoxiainduced lung injury in newborn rats, and the effect of glucocorticoid on this pathway. Methods Twenty-four Sprague- Dawley neonatal rats were randomly divided into three groups (n=8 each): sham control (control group), hyperoxiainduced acute lung injury (model group) and glucocorticoid-treated acute lung injury (glucocorticoid group). Rats were sacrificed at 13 days after birth. RAGE and NF-κB expression levels in lung tissues were detected by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry analysis. The levels of tumor necrosis factor α (TNF-α) and sRAGE in bronchoalveolar lavage fluid (BALF) and serum were measured using ELISA. Lung damage was evaluated by histological examinations. Results RAGE and NF-κB mRNA and protein expression levels in lung tissues were significantly increased in the model and glucocorticoid groups compared with the control group (P<0.05). Serum RAGE concentrations were significantly increased but RAGE concentrations in BALF were significantly reduced in the model and glucocorticoid groups compared with the control group (P<0.05). RAGE and NF-κB expression at both mRNA and protein levels in lung tissues was significantly lower in the glucocorticoid group than in the model group (P<0.05). RAGE concentrations were significantly lower in serum (P<0.05), but were higher in BALF (P<0.05) in the glucocorticoid group than in the model group. Conclusions RAGE-NF-κB pathway plays an important role in hyperoxia-induced lung injury in neonatal rats, and glucocorticoid administration may play a protective role against the lung injury by down-regulating RAGE-NF-κB signaling pathway.
Key wordsGlucocorticoid    Receptor for advanced glycation end-products    Lung injury    Neonatal rats   
收稿日期: 2014-06-06     
基金资助:江苏省临床医学专项(BL2014063).
通讯作者: 田兆方,男,主任医师.   
作者简介: 胡剑,男,硕士研究生,主治医师.
引用本文:   
胡剑,俞敏,唐云等. 糖皮质激素对高氧诱导新生大鼠肺组织RAGE-NF-κB通路的影响[J]. 中国当代儿科杂志, 2015, 17(1): 81-85.
HU Jian,YU Min,TANG Yun et al. Effects of glucocorticoid on RAGE-NF-κB pathway in hyperoxia-induced lung tissues of neonatal rats[J]. CJCP, 2015, 17(1): 81-85.
链接本文:  
http://www.zgddek.com/CN/10.7499/j.issn.1008-8830.2015.01.018     或     http://www.zgddek.com/CN/Y2015/V17/I1/81
版权所有 ©《中国当代儿科杂志》编辑部
地址:湖南省长沙市湘雅路87号 电话:0731-84327402  传真:0731-84327922 E-mail: cjcp1999@csu.edu.cn 
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn
湘ICP备17021739号-4