Abstract：Objective To investigate the association between CHI3L1 single nucleotide polymorphisms (SNPs) and the susceptibility to childhood asthma. Methods A total of 316 children diagnosed with asthma between January 2011 and October 2013 and 297 healthy children were selected as asthma group and control group respectively. Peripheral blood samples were collected from all subjects. Chemiluminescence and flow cytometry were applied to measure total IgE level and the percentage of eosinophils. ELISA was used to measure YKL-40 level. Genomic DNA was extracted from peripheral blood hemocytes, and the genotype and allele frequencies at CHI3L1 SNPs rs4950928, rs10399805, and rs883125 were determined by MALDI-TOP mass spectrometry. Results The total IgE and YKL-40 levels were significantly higher in the asthma group than in the control group (PP>0.05). The frequency of GG genotype at rs883125 in the asthma group was significantly higher than that in the control group (PPPPConclusions YKL-40 is a potential molecular biomarker for the primary diagnosis of childhood asthma. CHI3L1 SNPs rs4950928 and rs883125 may be associated with childhood asthma. G allele at rs4950928 may increase the risk of childhood asthma.
Devries A, Vercelli D. Epigenetics of human asthma and allergy: promises to keep[J]. Asian Pac J Allergy Immunol, 2013, 31(3): 183-189.
[4]
Zhao G, Lin X, Zhou M, et al. Association between CC10 +38A/G polymorphism and asthma risk: a meta-analysis[J]. Pak J Med Sci, 2013, 29(6): 1439-1443.
[5]
Hong JH, Hong JY, Park B, et al. Chitinase activates protease-activated receptor-2 in human airway epithelial cells[J]. Am J Respir Cell Mol Biol, 2008, 39(5): 530-535.
[6]
Chupp GL, Lee CG, Jarjour N, et al. A chitinase-like protein in the lung and circulation of patients with severe asthma[J]. N Engl J Med, 2007, 357(20): 2016-2027.
[7]
Kjaergaard AD, Johansen JS, Nordestgaard BG, et al. Genetic variants in CHI3L1 influencing YKL-40 levels: resequencing 900 individuals and genotyping 9000 individuals from the general population[J]. J Med Genet, 2013, 50(12): 831-837.
Sohn MH, Lee JH, Kim KW, et al. Genetic variation in the promoter region of chitinase 3-like 1 is associated with atopy[J]. Am J Respir Crit Care Med, 2009, 179(6): 449-456.
[10]
Fontana RJ, Litman HJ, Dienstag JL, et al. For the HALT-C Trial Group YKL-40 genetic polymorphisms and the risk of liver disease progression in patients with advanced fibrosis due to chronic hepatitis C[J]. Liver Int, 2012, 32(4): 665-674.
[11]
Zheng JL, Lu L, Hu J, et al. Genetic polymorphisms in chitinase 3-like 1 (CHI3L1) are associated with circulating YKL-40 levels, but not with angiographic coronary artery disease in a Chinese population[J]. Cytokine, 2011, 54(1): 51-55.
[12]
Konradsen JR, James A, Nordlund B, et al. The chitinase-like protein YKL-40: a possible biomarker of inflammation and airway remodeling in severe pediatric asthma[J]. J Allergy Clin Immunol, 2013, 132(2): 328-335.
[13]
Ortega H, Prazma C, Suruki RY, et al. Association of CHI3L1 in African-Americans with prior history of asthma exacerbations and stress[J]. J Asthma, 2013, 50(1): 7-13.
[14]
Rathcke CN, Holmkvist J, Husmoen LL, et al. Association of polymorphisms of the CHI3L1 gene with asthma and atopy: a populations-based study of 6514 Danish adults[J]. PLoS One, 2009, 4(7): e6106.
[15]
Yamamori H, Hashimoto R, Ohi K, et al. A promoter variant in the chitinase 3-like 1 gene is associated with serum YKL-40 level and personality trait[J]. Neurosci Lett, 2012, 513(2): 204-208.
