Abstract：Objective To study the characteristics of the colonization of 8 species of bifidobacteria by systematically profiling fecal bifidobacterial community in the early life of infants. Methods Fresh fecal samples including meconium samples were collected for culture and isolation of fecal bifidobacteria from 16 cases of full-term newborn infants born between March and April 2013 at their life of 2, 4, 7, 10, 14, 28, and 90 days. The isolated fecal bifidobacteria were taxonomically identified to genus and 8 species with PCR analysis. Results One hundred and fiftytwo predominant bifidobacteria strains were detected in the fecal samples, the detection rate of B. breve (22.4%) were the highest. Bifidobacteria were found in the feces of 8% infants 4 days after birth. The colonization rates increased to 54% and 60% at 28 days and 3 months respectively, significantly exceeding the colonization rate at 4 days after birth (P<0.05). Adult-type bifidobacteria B. catenulatum were found in the infants 10 days after birth, and infant-type bifidobacteria B. infantis were found at 14 days after birth, but infant-type bifidobacteria B. infantis were detected at a high level until 3 months after birth. The most tested infants had 2 species or less of bifidobacteria. Conclusions Intestinal bifidobacteria in infants might have less diversity in early infancy. Infant-type bifidobacteria appear late, while adult-type bifidobacteria colonize earlier.
Rautava S, Luoto R, Salminen S, et al. Microbial contact during pregnancy, intestinal colonization and human disease[J]. Nat Rev Gastroenterol Hepatol, 2012, 9(10): 565-576.
[2]
Sjogren YM, Tomicic S, Lundberg A, et al. Influence of early gut microbiota on the maturation of childhood mucosal and systemic immune responses[J]. Clin Exp Allergy, 2009, 39(12): 1842-1851.
Penders J, Thijs C, Vink C, et al. Factors influencing the composition of the intestinal microbiota in early infancy[J]. Pediatrics, 2006, 118(2): 511-521.
[6]
Mitsuoka T. A color atlas of anaerobic bacteria[M]. Tokyo: Sobunsya, 1980: 341.
[7]
Matsuki T, Watanabe K, Fujimoto J, et al. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria[J]. Appl Environ Microbiol, 2004, 70(1): 167-173.
[8]
Roberts AK, Chierici R, Sawatzki G, et al. Supplementation of an adapted formula with bovine lactoferrin: 1. Effect on the infant faecal flora[J]. Acta Paediatrica, 1992, 81(2): 119-124.
Azad MB, Konya T, Maughan H, et al. Gut microbiota of healthy Canadian infants: profiles by mode of delivery and infant diet at 4 months[J]. CMAJ, 2013, 185(5): 385-394.
[12]
He F, Ouwehand AC, Isolauri E, et al. Comparison of mucosal adhesion and species identification of bifidobacteria isolated from healthy and allergic infants[J]. FEMS Immunol Med Microbiol, 2001, 30(1): 43-47.
[13]
Favier CF, de Vos WM, Akkermans ADL. Development of bacterial and bifidobacterial communities in feces of newborn babies[J]. Anaerobe, 2003, 9(5): 219-229.
[14]
Biasucci G, Benenati B, Morelli L, et al. Cesarean delivery may affect the early biodiversity of intestinal bacteria[J]. J Nutr, 2008, 138(9): 1796S-1800S.
[15]
Gronlund MM, Lehtonen OP, Eerola E, et al. Fecal microflora in healthy infants born by different methods of delivery: permanent changes in intestinal flora after cesarean delivery[J]. J Pediatr Gastroenterol Nutr, 1999, 28(1): 19-25.
[16]
Avershina E, Storro O, Oien T, et al. Bifidobacterial succession and correlation networks in a large unselected cohort of mothers and their children[J]. Appl Environ Microbiol, 2013, 79(2): 497-507.
[17]
Tsuji H, Oozeer R, Matsuda K, et al. Molecular monitoring of the development of intestinal microbiota in Japanese infants[J]. Benef Microbes, 2012, 3(2): 113-125.
[18]
Matsuki T, Watanabe K, Tanaka R, et al. Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers[J]. Appl Environ Microbiol, 1999, 65(10): 4506.
[19]
Mullie C, Romond MB, Izard D. Establishment and follow-up of bifidobacterial species in the gut of healthy bottle-fed infants of 1-4 months age[J]. Folia Microbiol, 2006, 51(5): 473-477.
