Percentages of peripheral blood γδ T cells and regulatory T cells and expression of associated cytokines in infants with human cytomegalovirus infection
XU Ling, ZHU Li-Li, YE Li-Li, MENG Ling-Jian, LIU Wen-Qiang, WANG Jun
Department of Pediatrics, Affiliated Hospital, Xuzhou Medical University, Xuzhou, Jiangsu 221000, China
Abstract：Objective To investigate the percentages of peripheral blood γδ T cells and regulatory T cells (Treg) and the expression of associated cytokines, interleukin 17 (IL-17) and transforming growth factor-β1 (TGF-β1), in infants with human cytomegalovirus (HCMV) infection. Methods Twenty-two infants with HCMV infection (HCMV group) and 22 healthy infants who underwent physical examination (control group) were enrolled in this study. The percentages of peripheral blood γδ T cells and Treg cells were determined by flow cytometry. The levels of IL-17 and TGF-β1 in plasma were measured using ELISA. Results Compared with the control group, the HCMV group had significantly higher percentage of γδ T cells and IL-17 level (P < 0.01) and significantly lower percentage of Treg cells and TGF-β1 level (P < 0.01). In the HCMV group, the percentage of γδ T cells was negatively correlated with the percentage of Treg cells and TGF-β1 level (P < 0.05), but positively correlated with IL-17 level (P < 0.05); the percentage of Treg cells was positively correlated with TGF-β1 level (P < 0.05), but negatively correlated with IL-17 level (P < 0.05); there was no correlation between IL-17 level and TGF-β1 level (P > 0.05). Conclusions There is an imbalance between γδ T cells and Treg cells in the peripheral blood of infants with HCMV infection, and γδ T cells may be involved in the secretion of IL-17.
XU Ling,ZHU Li-Li,YE Li-Li et al. Percentages of peripheral blood γδ T cells and regulatory T cells and expression of associated cytokines in infants with human cytomegalovirus infection[J]. CJCP, 2018, 20(3): 204-208.
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