学龄双生子儿童焦虑抑郁与5-HTTLPR基因多态性的关系

赵乔; 李玉玲; 恩和巴雅尔; 刘燕; 丁一

doi:10.7499/j.issn.1008-8830.2016.01.013

PDF(1538 KB)

HTML

中国当代儿科杂志 ›› 2016, Vol. 18 ›› Issue (1) : 61-66. DOI: 10.7499/j.issn.1008-8830.2016.01.013

论著·临床研究

学龄双生子儿童焦虑抑郁与5-HTTLPR基因多态性的关系

赵乔, 李玉玲, 恩和巴雅尔, 刘燕, 丁一

作者信息 +

Association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins

ZHAO Qiao, LI Yu-Ling, Enherbayaer, LIU Yan, DING Yi

Author information +

文章历史 +

摘要

目的了解5-HTTLPR 基因多态性与学龄双生子儿童焦虑抑郁的关系。方法选取内蒙古呼和浩特市和包头市8~12 岁147 对双生子儿童(同卵双生子47 对;异卵双生子100 对)为调查对象,运用Achenbach 儿童行为量表(CBCL)测算儿童焦虑抑郁因子得分;采集所有儿童口腔上皮细胞并提取DNA,运用聚合酶链式反应(PCR)方法对5-HTTLPR 基因进行分型;利用广义估计方程(GEE)分析5-HTTLPR 基因多态性及家庭环境因素对学龄双生子儿童焦虑抑郁的影响。结果 LS 和SS 基因型儿童的焦虑抑郁因子得分高于LL 基因型儿童(χ²=3.938,P<0.05);5-HTTLPR 基因型与家庭亲密度(χ²=6.129,P<0.05)和家庭教养方式(χ²=7.665,P<0.05)的交互作用对儿童焦虑抑郁因子得分影响显著。结论 5-HTTLPR 基因型与学龄双生子儿童焦虑抑郁因子得分显著相关;在高亲密度和专制型教养方式的家庭环境中,S 等位基因可能增加双生子儿童焦虑抑郁的可能性。

Abstract

Objective To investigate the association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins. Methods A total of 147 pairs of twins (47 pairs of monozygotic twins, 100 pairs of dizygotic twins) aged 8-12 years from Baotou and Hohhot were selected as respondents. The Achenbach Child Behavior Checklist (CBCL) was used to calculate the scores of anxiety-depression factors in school-aged twins. The DNA was extracted from oral epithelial cells, and polymerase chain reaction was applied for 5-HTTLPR genotyping. The generalized estimating equation (GEE) was used to analyze the effect of 5-HTTLPR polymorphism and family environment on anxiety-depression in school-aged twins. Results The children with LS and SS genotypes had significantly higher scores of anxiety-depression factors than those with LL genotype (χ²=3.938, P<0.05). The interaction of 5-HTTLPR genotype with family cohesion and family rearing patterns had a significant impact on the scores of anxiety-depression factors in twins (χ²=6.129 and 7.665, both P<0.05). Conclusions 5-HTTLPR genotype is significantly correlated with the scores of anxiety-depression factors in school-aged twins. In the family with high cohesion and an autocratic family rearing pattern, S allele may increase the possibility of anxiety-depression in twin children.

导出引用

赵乔, 李玉玲, 恩和巴雅尔, 刘燕, 丁一. 学龄双生子儿童焦虑抑郁与5-HTTLPR基因多态性的关系[J]. 中国当代儿科杂志. 2016, 18(1): 61-66 https://doi.org/10.7499/j.issn.1008-8830.2016.01.013

ZHAO Qiao, LI Yu-Ling, Enherbayaer, LIU Yan, DING Yi. Association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(1): 61-66 https://doi.org/10.7499/j.issn.1008-8830.2016.01.013

参考文献

[1] Martine B, Anne D, Jean-Luc R. The French version of the Revised Child Anxiety and Depression Scale (RCADS) in a nonclinical sample[J]. Swiss J Psychol, 2015, 74(3): 119-127.
[2] Pickles A, Hill J, Breen G, et al. Evidence for interplay between genes and parenting on infant temperament in the first year of life: monoamine oxidase A polymorphism moderates effects of maternal sensitivity on infant anger proneness[J]. J Child Psychol Psychiatry, 2013, 54(12): 1308-1317.
[3] Banducci AN, Gomes M, MacPherson L, et al. A Preliminary examination of the relationship between the 5-HTTLPR and childhood emotional abuse on depressive symptoms in 10-12-year-old youth[J]. Psychol Trauma, 2014, 6(1): 1-7.
[4] 李玉玲, 季成叶, 关宏岩, 等. 学龄儿童行为遗传双生子研究[J]. 中国学校卫生, 2012, 33(9): 1033-1037.
[5] Lesch KP, Bengel D, Heils A, et al. Association of anxietyrelated traits with a polymorphism in the serotonin transporter gene regulatory region[J]. Science, 1996, 274(5292): 1527-1531.
[6] Pezawas L, Meyer-Lindenberg A, Drabant EM, et al. 5-HTTLPR polymorphism impacts human cingulate-amygdala interactions: a genetic susceptibility mechanism for depression[J]. Nat Neurosci, 2005, 8(6): 828-834.
[7] 陈羿君, 陈翠, 魏冰思, 等. 学习困难儿童焦虑情绪与行为问题影响因素分析[J]. 中国公共卫生2014, 30(7): 853-857.
[8] 李静. 抽动障碍儿童焦虑抑郁情绪及影响因素的研究[D]. 大连: 大连医科大学, 2013.
[9] 侯金芹, 陈祉妍, 李新影, 等. 青少年抑郁情绪的行为遗传学研究[J]. 心理学报, 2012, 44(10): 1329-1338.
[10] 汪向东, 王希林, 马弘. 心理卫生评定量表手册[M]. 北京: 中国心理卫生杂志社, 1999: 49-52.
[11] 费立鹏, 沈其杰, 郑延平, 等. “家庭亲密度和适应性量表” 和“家庭环境量表”的初步评价--正常家庭与精神分裂症家庭成员对照研究[J]. 中国心理卫生杂志, 1991, 5(5): 198-202.
[12] 岳冬梅, 李鸣杲, 金魁和, 等. 父母教养方式: EMBU 的初步修订及其在神经症患者的应用[J]. 中国心理卫生杂志, 1993, 7(3): 97-101.
[13] Heils A, Teufel A, Petri S, et al. Allelic variation of human serotonin transporter gene expression[J]. J Neurochem, 1996, 66(6): 2621-2624.
[14] Collier DA, Stöber G, Li T, et al. A novel functional polymorphism within the promoter of the serotonin transporter gene: possible role in susceptibility to affective disorders[J]. Mol Psychiatry, 1996, 1(6): 453-460.
[15] Hoefgen B, Schulze TG, Ohlraun S, et al. The power of sample size and homogenous sampling: association between the 5-HTTLPR serotonin transporter polymorphism and major depressive disorder[J]. Biol Psychiatry, 2005, 57(3): 247-251.
[16] Auerbach J, Geller V, Lezer S, et al. Dopamine D4 receptor (D4DR) and serotonin transporter promoter (5-HTTLPR) polymorphisms in the determination of temperament in 2-month-old-infants[J]. Mol Psychiatry, 1999, 4(4): 369-373.
[17] Hayden EP, Dougherty LR, Maloney B. Temperamental fearfulness in childhood and the serotonin transporter promoter region polymorphism: a multimethod association study[J]. Psychiatr Genet, 2007, 17(3): 135-142.
[18] Lesch KP, Gross J, Franzek E, et al. Primary structure of the serotonin transporter in unipolar depression and bipolar disorder[J]. Biol Psychiatry, 1995, 37(4): 215-223.
[19] 杜岸政. 家庭亲密度和适应性、父母自我分化与儿童品行问题的关系研究[J]. 中国儿童保健杂志, 2015, 23(5): 475-477.
[20] 沈玲, 罗学荣, 韦臻, 等. 焦虑性障碍儿童家庭环境因素的研究[J]. 中国儿童保健杂志, 2011, 19(10): 897-899.
[21] 王美芳, 张燕翎, 于景凯, 等. 幼儿焦虑与气质、家庭环境的关系[J]. 中国临床心理学杂志, 2012, 20(3): 371-397.
[22] 朱庆庆, 古桂雄, 花静. 注意缺陷多动障碍儿童家庭环境危险因素研究[J]. 中国妇幼保健, 2015, 30(7): 1074-1077.
[23] 王美芳, 张燕翎, 于景凯, 等. 幼儿焦虑与气质、家庭环境的关系[J]. 中国临床心理学杂志, 2012, 20(3): 371-373.
[24] 张亚峰, 孙桂香. 儿童注意缺陷多动障碍家庭危险因素的 Meta 分析[J]. 中国当代儿科杂志, 2015, 17(7): 721-725.
[25] 朱明哲, 朱晓晶, 杜建娥, 等. 父母短期教育干预对学龄前儿童焦虑的影响[J]. 中国当代儿科杂志, 2014, 16(9): 901-904.
[26] 曹玉萍, 李龙飞, 赵幸福, 等. 儿童攻击行为与COMT Val158Met 及5-HTTLPR 基因多态性关联分析[J]. 中国当代儿科杂志, 2011, 13(5): 361-364.
[27] 恩和巴雅尔, 李玉玲, 关宏岩, 等. DRD2 基因rs1079595 多态性对学龄儿童注意分散度的影响[J]. 中国当代儿科杂志, 2015, 17(3): 270-274.