目的 测定学龄前哮喘儿童外周血中性粒细胞弹性蛋白酶(NE)水平,阐明NE在哮喘急性发作诊断中的作用。方法 收集2008年1月至2010年1月确诊为哮喘的学龄前儿童85例,分为哮喘急性发作组44例(急性组)和非急性发作组41例(非急性组);同期35例健康体检儿童作为对照组。用ELISA法检测各组外周血的NE和白细胞介素-8(IL-8)水平,并进行NE的受试者工作特征曲线(ROC)评价。结果 哮喘组的NE和IL-8水平高于对照组,其中急性发作组高于非急性发作组(P<0.05);NE和IL-8水平呈正相关(r=0.48,P<0.01)。以NE水平>27.73μg/L作为判断哮喘急性发作的界值,其灵敏度为65.9%、特异度为95.1%,ROC曲线下面积为0.87(P<0.01)。结论 通过检测学龄前哮喘儿童NE水平,有助于哮喘急性发作的诊断。
Abstract
Objective To study the role of serum neutrophil elastase (NE) level in acute exacerbation of asthma in preschool children. Methods A total of 85 preschool children who were diagnosed with asthma between January 2008 and January 2010 were classified into acute exacerbation group (n=44) and non-acute exacerbation group (n=41). Thirty-five children who received physical examination served as the control group. The enzyme-linked immunosorbent assay was used to determine the serum levels of NE and interleukin-8 (IL-8). The receiver operating characteristic (ROC) curve was used for NE evaluation. Results Both the acute and non-acute exacerbation groups had higher serum levels of NE and IL-8 than the control group, and the acute exacerbation group had significantly higher serum levels of NE and IL-8 than the non-acute exacerbation group (P<0.05). The serum level of NE was positively correlated with that of IL-8 (r=0.48, P<0.05). With serum NE level >27.73 μg/L as the cut-off value for diagnosing acute exacerbation of asthma, the sensitivity was 65.9%, the specificity was 95.1%, and the area under the ROC curve was 0.87 (P<0.01). Conclusions The determination of serum NE level in preschool children with asthma helps to diagnose the acute exacerbation of asthma.
关键词
哮喘 /
中性粒细胞弹性蛋白酶 /
受试者工作特征曲线 /
学龄前儿童
Key words
Asthma /
Neutrophil elastase /
Receiver operating characteristic curve /
Preschool child
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基金
无锡市医院管理中心医学科研重点研究项目(编号YGZXZ1530)。
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