生物标记物粪便胆汁酸的测定在过敏性紫癜患儿诊治中的意义

胡红卫; 段江; 赵波; 熊晶晶; 刘梅; 崔晶晶; 吉晓菲; 张婷婷; 张丽芝; 黄永坤

doi:10.7499/j.issn.1008-8830.2016.06.010

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中国当代儿科杂志 ›› 2016, Vol. 18 ›› Issue (6) : 517-521. DOI: 10.7499/j.issn.1008-8830.2016.06.010

论著·临床研究

生物标记物粪便胆汁酸的测定在过敏性紫癜患儿诊治中的意义

胡红卫¹, 段江¹, 赵波², 熊晶晶¹, 刘梅¹, 崔晶晶², 吉晓菲¹, 张婷婷¹, 张丽芝¹, 黄永坤¹

作者信息 +

Significance of detection of biomarker fecal bile acids in the diagnosis and treatment of childhood Henoch-Schönlein purpura

HU Hong-Wei¹, DUAN Jiang¹, ZHAO Bo², XIONG Jing-Jing¹, LIU Mei¹, CUI Jing-Jing², JI Xiao-Fei¹, ZHANG Ting-Ting¹, ZHANG Li-Zhi¹, HUANG Yong-Kun¹

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摘要

目的探讨生物标记物粪便胆汁酸浓度在过敏性紫癜(HSP)患者中的变化及其在诊治中的临床意义。方法选取2014~2016年确诊为HSP的19例患儿为HSP组,另选取27例健康儿童为健康对照组。采集HSP组患儿急性期、恢复期及健康对照组儿童粪便标本,应用液相质谱技术检测各组儿童粪便胆汁酸水平。结果 HSP组患儿恢复期胆酸水平均高于健康对照组和HSP组急性期 (P < 0.016)。HSP组患儿恢复期鹅脱氧胆酸水平高于健康对照组 (P < 0.016)。HSP组患儿急性期和恢复期脱氧胆酸、石胆酸水平均低于健康对照组 (分别P < 0.05、P < 0.016)。各组间熊去氧胆酸水平比较差异均无统计学意义 (P > 0.05)。结论 HSP患儿急性期粪便次级胆汁酸脱氧胆酸和石胆酸低于健康对照组,这可能与HSP的发病或转归有关。

Abstract

Objective To investigate the changes and clinical significance of biomarker fecal bile acids (BA) in children with Henoch-Schönlein purpura (HSP). Methods Nineteen children with HSP and twenty-seven healthy children were enrolled in this study. The stool samples were obtained at the acute and remission phases. Fecal BA levels were measured by high performance liquid chromatography mass spectrometry (HPLC-MS). Results The fecal cholic acid level in the HSP remission group was significantly higher than in the HSP acute group and the healthy control group (P < 0.016). The fecal chenodeoxycholic acid level in the HSP remission group was significantly higher than in the healthy control group (P < 0.016). The levels of fecal secondary colonic bile acids, deoxycholic acid and lithocholic acid, in the HSP acute and remission groups were significantly lower than in the healthy control group(P < 0.05, P < 0.016 respectively). No significant differences were found in the levels of fecal urosodeoxycholic acid among the three groups (P > 0.05). Conclusions Fecal secondary colonic bile acids, deoxycholic acid and lithocholic acid, are in decrease in children with HSP at the acute stage, which may be involved in the pathogenesis and treatment outcomes of HSP.

导出引用

胡红卫, 段江, 赵波, 熊晶晶, 刘梅, 崔晶晶, 吉晓菲, 张婷婷, 张丽芝, 黄永坤. 生物标记物粪便胆汁酸的测定在过敏性紫癜患儿诊治中的意义[J]. 中国当代儿科杂志. 2016, 18(6): 517-521 https://doi.org/10.7499/j.issn.1008-8830.2016.06.010

HU Hong-Wei, DUAN Jiang, ZHAO Bo, XIONG Jing-Jing, LIU Mei, CUI Jing-Jing, JI Xiao-Fei, ZHANG Ting-Ting, ZHANG Li-Zhi, HUANG Yong-Kun. Significance of detection of biomarker fecal bile acids in the diagnosis and treatment of childhood Henoch-Schönlein purpura[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(6): 517-521 https://doi.org/10.7499/j.issn.1008-8830.2016.06.010

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