Abstract：Objective To investigate the changes in the proportion and function of peripheral CD4+LAP+regulatory T cells (CD4+LAP+Treg cells) in children with asthma, as well as the role of CD4+LAP+Treg cells in the pathogenesis of asthma. Methods A total of 75 children who were diagnosed with asthma from March 2014 to September 2015 were enrolled as study subjects, and according to their conditions, they were divided into acute-stage asthma group (40 children) and remission-stage asthma group (35 patients). Another 30 children who underwent physical examination were enrolled as the healthy control group. Flow cytometry was used to determine the percentage of peripheral CD4+LAP+Treg cells, and[3H]-thymidine incorporation assay was performed to analyze the immunosuppression of CD4+LAP+Treg cells in each group. Results The acute-stage asthma group showed significant reductions in the proportion of CD4+LAP+Treg cells compared with the remission-stage asthma group and the healthy control group (2.0%±1.0% vs 4.1%±2.4%/4.6%±3.0%; P +LAP+Treg cells compared with the remission-stage asthma group and the healthy control group (21%±4% vs 55%±9%/62%±11%; P Conclusions In children with asthma, the reduction in the number and inhibitory function of peripheral CD4+LAP+Treg cells may be involved in the pathogenesis of asthma.
LUO Jing-Hua,YANG Liang-An,LI Guo-Lin. Changes in proportion and function of peripheral CD4+LAP+ regulatory T cells in children with asthma[J]. CJCP, 2016, 18(8): 726-730.
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