Abstract：Objective To investigate the relationship of KI polyomavirus (KIPyV) and WU polyomavirus (WUPyV) with acute respiratory infection in children in Tianjin, China. Methods A total of 3 730 nasopharyngeal secretions were collected from hospitalized children with acute respiratory infection in Tianjin Children's Hospital from January 2011 to December 2013. Viral nucleic acid was extracted, and virus infection (KIPyV and WUPyV) was determined by PCR. Some KIPyV-positive and WUPyV-positive PCR products were subjected to sequencing. Sequencing results were aligned with the known gene sequences of KIPyV and WUPyV to construct a phylogenetic tree. Amplifed VP1 fragments of KIPyV were inserted into the cloning vector (PUCm-T) transformed into E. coli competent cells. Positive clones were identifed by PCR and sequencing. The nucleotide sequences were submitted to GenBank. In addition, another seven common respiratory viruses in all samples were detected by direct immunofluorescence assay. Results In the 3 730 specimens, the KIPyV-positive rate was 12.14% (453/3 730) and the WUPyV-positive rate was 1.69% (63/3730). The mean infection rate of KIPyV was signifcantly higher in June and July, while the mean infection rate of WUPyV peaked in February and March. Most of the KIPyV-positive or WUPyV-positive children were < 3 years. The co-infections with KIPyV, WUPyV, and other respiratory viruses were observed in the children. The coinfection rate was 2.31% (86/3 730) and there were nine cases of co-infections with WUPyV and KIPyV. Thirty-fve KIPyV-positive and twelve WUPyV-positive PCR products were sequenced and the alignment analysis showed that they had high homology with the known sequences (94%-100% vs 95%-100%). The VP1 gene sequences obtained from two KIPyV strains in this study were recorded in GenBank with the accession numbers of KY465925 and KY465926. Conclusions For some children with acute respiratory infection in Tianjin, China, the acute respiratory infection may be associated with KIPyV and WUPyV infections. KIPyV infection is common in summer, and WUPyV infection in spring. The epidemic strains in Tianjin have a high homology with those in other regions.
LIN Shu-Xiang,WANG Wei,GUO Wei et al. A molecular epidemiological study of KI polyomavirus and WU polyomavirus in children with acute respiratory infection in Tianjin, China[J]. CJCP, 2017, 19(7): 763-769.
Mulholland K. Global burden of acute respiratory infections in children:implications for interventions[J]. Pediatr Pulmonol, 2003, 36(6):469-474.
[2]
Lowther SA, Shay DK, Holman RC, et al. Bronchiolitisassociated hospitalizations among American Indian and Alaska Native children[J]. Pediatr Infect Dis J, 2000, 19(1):11-17.
[3]
Gaynor AM, Nissen MD, Whiley DM, et al. Identifcation of a novel polyomavirus from patients with acute respiratory tract infections[J]. PLoS Pathog, 2007, 3(5):e64.
[4]
Gardner SD, Field AM, Coleman DV, et al. New human papovavirus (B.K.) isolated from urine after renal transplantation[J]. Lancet, 1971, 1(7712):1253-1257.
[5]
Padgett BL, Walker DL, Zurhein GM, et al. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy[J]. Lancet, 1971, 1(7712):1257-1260.
[6]
Allander T, Andreasson K, Gupta S, et al. Identification of a third human polyomavirus[J]. J Virol, 2007, 81(8):4130-4136.
[7]
Zhuang WL, Lu XD, Lin GY, et al. WU polyomavirus infection among children in South China[J]. J Med Virol, 2011, 83(8):1440-1445.
[8]
Debiaggi M, Canducci F, Brerra R, et al. Molecular epidemiology of KI and WU polyomaviruses in infants with acute respiratory disease and in adult hematopoietic stem cell transplant recipients[J]. J Med Virol, 2010, 82(1):153-156.
[9]
Okada M, Hamada H, Sato-Maru H, et al. WU polyomavirus detected in respiratory tract specimens from young children in Japan[J]. Pediatr Int, 2013, 55(4):536-537.
[10]
Falcone V, Panning M, Strahm B, et al. Prolonged KI polyomavirus infection in immunodefcient child[J]. Emerg Infect Dis, 2012, 18(4):706-708.
[11]
Hormozdi DJ, Arens MQ, Le BM, et al. KI polyomavirus detected in respiratory tract specimens from patients in St. Louis, Missouri[J]. Pediatr Infect Dis J, 2010, 29(4):329-333.
[12]
Foulongne V, Brieu N, Jeziorski E, et al. KI and WU polyomaviruses in children, France[J]. Emerg Infect Di, 2008, 14(3):523-525.
[13]
Abedi Kiasari B, Vallely PJ, Corless CE, et al. Age-related pattern of KI and WU polyomavirus infection[J]. J Clin Virol, 2008, 43(1):123-125.
[14]
van der Zalm MM, Rossen JW, van Ewijk BE, et al. Prevalence and pathogenicity of WU and KI polyomaviruses in children, the Netherlands[J]. Emerg Infect Dis, 2008, 14(11):1787-1789.
[15]
Bialasiewicz S, Whiley DM, Lambert SB, et al. Presence of the newly discovered human polyomaviruses KI and WU in Australian patients with acute respiratory tract infection[J]. J Clin Virol, 2008, 41(2):63-68.
[16]
Han TH, Chung JY, Koo JW, et al. WU polyomavirus in children with acute lower respiratory tract infections, South Korea[J]. Emerg Infect Dis, 2007, 13(11):1766-1768.
[17]
Yuan XH, Jin Y, Xie ZP, et al. Prevalence of human KI and WU polyomaviruses in children with acute respiratory tract infection in China[J]. J Clin Microbiol, 2008, 46(10):3522-3525.
[18]
Payungporn S, Chieochansin T, Thongmee C, et al. Prevalence and molecular characterization of WU/KI polyomaviruses isolated from pediatric patients with respiratory disease in Thailand[J]. Virus Res, 2008, 135(2):230-236.
[19]
Furuse Y, Suzuki A, Kishi M, et al. Detection of novel respiratory viruses from influenza-like illness in the Philippines[J]. J Med Virol, 2010, 82(6):1071-1074.
[20]
Teramoto S, Kaiho M, Takano Y, et al. Detection of KI polyomavirus and WU polyomavirus DNA by real-time polymerase chain reaction in nasopharyngeal swabs and in normal lung and lung adenocarcinoma tissues[J]. Microbiol Immunol, 2011, 55(7):525-530.
Ren L, Gonzalez R, Xie Z, et al. WU and KI polyomavirus present in the respiratory tract of children, but not in immunocompetent adults[J]. J Clin Virol, 2008, 43(3):330-333.
[27]
Mueller A, Simon A, Gillen J, et al. Polyomaviruses KI and WU in children with respiratory tract infection[J]. Arch Virol, 2009, 154(10):1605-1608.
Le BM, Demertzis LM, Wu G, et al. Clinical and epidemiologic characterization of WU polyomavirus infection, St. Louis, Missouri[J]. Emerg Infect Dis, 2007, 13(12):1936-1938.
[30]
Suzuki A, Lupisan S, Furuse Y, et al. Respiratory viruses from hospitalized children with severe pneumonia in the Philippines[J]. BMC Infect Dis, 2012, 12:267.
