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血管细胞间黏附分子-1在毛细支气管炎患儿中的表达及意义
Expression of vascular intercellular adhesion molecule-1 and its significance in children with bronchiolitis
目的 检测血管细胞间黏附分子-1（ICAM-1）在毛细支气管炎患儿血清和诱导痰上清液中的表达，探讨其在毛细支气管炎患儿发病中的作用和意义。方法 选取2015年7月至2017年1月确诊的毛细支气管炎住院患儿67例为研究对象，按诊断标准分为轻度组（n=22）、中度组（n=24）、重度组（n=21）。选取同期在我院行健康体检的20例婴幼儿为健康对照组。采用ELISA法检测各组毛细支气管炎患儿急性期及恢复期，以及健康对照组婴儿血清及诱导痰上清液中ICAM-1水平。结果 与健康对照组比较，急性期毛细支气管炎轻度组、中度组、重度组血清及痰液ICAM-1水平均显著升高（P < 0.01）；与轻度组比较，急性期中度组、重度组血清及痰液ICAM-1水平均显著升高（P < 0.01）；与中度组比较，急性期重度组血清及痰液ICAM-1水平显著升高（P < 0.01）；与毛细支气管炎各组患儿急性期比较，恢复期轻、中、重度组毛细支气管炎患儿血清及痰液ICAM-1水平均显著下降（P < 0.01）。相关性分析结果显示，毛细支气管炎患儿急性期痰液与血清ICAM-1水平呈正相关（r=0.875，P < 0.001）。结论 ICAM-1参与了毛细支气管炎的发病过程，且和病情严重程度相关。
Objective To investigate the expression of intercellular adhesion molecule-1 (ICAM-1) in serum and induced sputum supernatant in children with bronchiolitis, as well as its role in the pathogenesis of bronchiolitis in children.Methods A total of 67 children with bronchiolitis who were diagnosed and hospitalized between July 2015 and January 2017 were enrolled. According to the diagnostic criteria, these children were divided into mild group with 22 children, moderate group with 24 children, and severe group with 21 children. A total of 20 children who underwent physical examination were enrolled as healthy control group. ELISA was used to measure the level of ICAM-1 in serum and induced sputum supernatant in the children with bronchiolitis in the acute stage and recovery stage and the children in the healthy control group.Results Compared with the healthy control group, the mild, moderate, and severe bronchiolitis groups had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the mild group, the moderate and severe groups had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the moderate group, the severe group had a significant increase in the level of ICAM-1 in serum and sputum (P<0.01). Compared with the children with bronchiolitis in the acute stage, the children in the recovery stage had a significant reduction in the level of ICAM-1 in serum and sputum (P<0.01). The correlation analysis showed that in the acute stage, the level of ICAM-1 in serum was positively correlated with that in sputum in children with bronchiolitis (r=0.875,P<0.001).Conclusions ICAM-1 is involved in the pathogenesis of bronchiolitis and is associated with disease severity.
细胞间黏附分子-1 / 毛细支气管炎 / 血清 / 痰液 / 儿童
Intercellular adhesion molecule-1 / Bronchiolitis / Serum / Sputum / Child
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