目的 研究TOPK/PBK在恶性淋巴瘤与淋巴结反应性增生患儿淋巴结中的表达差异。方法 以80例恶性淋巴瘤、20例淋巴结反应性增生患儿为研究对象，应用免疫组化检测所有研究对象淋巴结组织的TOPK/PBK表达，对比分析TOPK/PBK的表达情况。结果 恶性淋巴瘤患儿的TOPK/PBK阳性率高于淋巴结反应性增生患儿（P < 0.05）；TOPK/PBK阳性率在霍奇金淋巴瘤（HL）与非霍奇金淋巴瘤（NHL）组的差异无统计学意义（P > 0.05）；淋巴母细胞淋巴瘤、成熟B细胞淋巴瘤、成熟T/NK细胞淋巴瘤的TOPK/PBK阳性率以淋巴母细胞淋巴瘤最高，但成熟B细胞淋巴瘤与成熟T/NK细胞淋巴瘤的阳性率差异无统计学意义（P > 0.016）。结论 TOPK/PBK在儿童恶性淋巴瘤的淋巴结组织中表达上调，其表达水平可能与NHL病理类型有关。

Objective To study the difference in expression of TOPK/PBK in lymph nodes between children with malignant lymphoma and those with reactive lymphoid hyperplasia. Methods Eighty children with malignant lymphoma and twenty children with reactive lymphoid hyperplasia were enrolled as subjects. Immunohistochemistry was used to determine the expression of TOPK/PBK in all the subjects. The expression of TOPK/PBK was compared between the two groups. Results The TOPK/PBK-positivity rate was significantly higher in children with malignant lymphoma than in those with reactive lymphoid hyperplasia (P < 0.05). There was no significant difference in the TOPK/PBK-positivity rate between the children with Hodgkin's lymphoma and non-Hodgkin's lymphoma (NHL). There were significant differences in the TOPK/PBK-positivity rate among children with different pathological types of NHL (P < 0.05):the children with lymphoblastic lymphoma showed the highest TOPK/PBK-positivity rate and those with mature B-cell lymphoma and mature T/NK-cell lymphoma had a similar TOPK/PBK-positivity rate. Conclusions The expression of TOPK/PBK is up-regulated in the lymph nodes of children with malignant lymphoma. The expression level of TOPK/PBK may be related to the pathological type of NHL.