INF-γ/A+874T位点基因多态性与呼吸道合胞病毒毛细支气管炎的相关性

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摘要目的：干扰素-γ(IFN-γ)分泌不足可能在严重呼吸道合胞病毒(RSV)感染的发病中发挥重要作用。IFN-γ/A+874T基因位点存在多态性，可能与RSV感染的病情存在关联。该研究分析INF-γ/A+874T基因位点在温州地区汉族儿童的分布特征；进一步探讨INF-γ/A+874T位点基因多态性与RSV毛细支气管炎易感性、病情的相关性及对鼻咽分泌物 INF-γ和血清总IgE水平的影响。方法：采用DNA测序法检测114例住院的汉族RSV毛细支气管炎患儿和90例汉族健康体检儿童INF-γ/A+874T位点基因多态性；采用酶联免疫吸附法测定鼻咽分泌物INF-γ水平，化学发光法测定血清总IgE水平。结果：两组均存在INF-γ/A+874T位点基因多态性，以AA纯合子基因型为主，其中RSV毛细支气管炎组AA、AT基因型频率分别为82.5%，17.5%，对照组分别为AA 77.8%，AT 21.1%，两组差异无显著性意义；病例组等位基因频率分别为A 90.4%，T 9.6%，对照组分别为A 88.3%，T 11.7%，两组差异均无显著性意义。轻度和中重度患儿INF-γ/+874位点2种基因型的差异亦无显著性意义。病例组2种基因型鼻咽分泌物INF-γ及血清总IgE水平差异均无显著性意义。结论：温州地区汉族儿童存在INF-γ/A+874T位点基因多态性，但未发现该位点变异与RSV毛细支气管炎易感性、病情、鼻咽分泌物INF-γ及血清总IgE水平存在关联。［中国当代儿科杂志，2009，11（1）：21-24］

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	董琳
	黄志英
	陈小芳
	周晓聪
	李锦燕
	林剑

关键词 ：呼吸道合胞病毒, 毛细支气管炎, 干扰素-γ, 基因多态性, 儿童

Abstract：OBJECTIVE: A deficient interferon-gamma (IFN-gamma) response has been involved in the pathogenesis of severe respiratory syncytial virus (RSV) infection. Gene polymorphisms in IFN-gamma/A+874T have been associated with the susceptibility to asthma and might be related to disease severity of RSV infection. This study investigated the single nucleotide polymorphisms (SNPs) of IFN-gamma/A+874T in Han children in Wenzhou area and to explore the correlation between gene polymorphisms of IFN-gamma/A +874T and the susceptibility and disease severity of RSV bronchiolitis, as well as the effect of SNPs upon nasopharyngeal secretions (NPS) IFN-gamma and total serum IgE levels. METHODS: One hundred and fourteen hospitalized children with RSV bronchiolitis and 90 healthy controls were recruited. Sequence analysis was used for detecting the SNPs of IFN-gamma/A+874T. NPS IFN-gamma levels were measured using ELISA. Total serum IgE levels were assayed using the chemiluminescence method. RESULTS: IFN-gamma/A+874T gene polymorphisms were present in both the patient and the control groups. AA and AT genotypes were found in both groups, with a AA frequency of 82.5% vs 77.8% and a AT frequency of 17.5% vs 21.1% (P>0.05). The frequency of allele was 90.4% (A) and 9.6% (T) in the patient group, and 88.3% (A) and 11.7% (T) in the control group, respectively. There were no significant differences in the allele frequency between the two groups. Moreover, no difference was found both in NPS IFN-gamma and total serum IgE levels between AA and AT genotypes in the patient group. There were no significant differences in the variation of IFN-gamma/+874 between mild and moderate to severe cases. CONCLUSIONS: IFN-gamma/A+874T gene polymorphisms were present in Han children in Wenzhou area. Gene variations were not associated with the susceptibility and disease severity of RSV bronchiolitis as well as IFN-gamma and total serum IgE levels.［Chin J Contemp Pediatr, 2009, 11 (1):21-24］

Key words： Respiratory syncytial virus Bronchiolitis Interferon-gamma Gene Polymorphisms Child

PACS:

R373.1

引用本文:

董琳,黄志英,陈小芳等. INF-γ/A+874T位点基因多态性与呼吸道合胞病毒毛细支气管炎的相关性[J]. 中国当代儿科杂志, 2009, 11(01): 21-24.

DONG Lin,HUANG Zhi-Ying,CHEN Xiao-Fang et al. Association of INF-γ/A+874T gene polymorphisms with respiratory syncytial virus bronchiolitis[J]. CJCP, 2009, 11(01): 21-24.

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http://www.zgddek.com/CN/ 或 http://www.zgddek.com/CN/Y2009/V11/I01/21

	［1］Pérez-Yarza EG, Moreno A, Lázaro P, Mejías A, Ramilo O. The association between respiratory syncytial virus infection and the development of childhood asthma: a systematic review of the literature［J］. Pediatr Infect Dis J, 2007, 26 (8):733-739.
	［2］Sigurs N, Gustafsson PM, Bjarnason R, Lundberg F, Schmidt S, Sigurbergsson F, et al. Severe respiratory syncytial virus bronchiolitis in infancy and asthma and allergy at age 13［J］. Am J Respir Crit Care Med, 2005, 171(1): 137-141.
	［3］Schauer U, Hoffjan S, Rothoeft T, Bartz H, Konig S, Fuchs E, et al. Severe respiratory syncytial virus infections and reduced interferon-gamma generation in vitro［J］.Clin Exp Immunol, 2004, 138(1):102-109.
	［4］Legg JP, Hussain IR, Warner JA, Johnston SL, Warner JO. Type1 and type 2 cytokine imbalance in acute respiratory syncytial virus bronchiolitis［J］. Am J Respir Crit Care Med, 2003, 168(6):633-639.
	［5］Tangwattanachuleeporn M, Sodsai P, Avihingsanon Y, Wongpiyabovorn J, Wongchinsri J, Hirankarn N. Association of interferon-gamma gene polymorphism (+874A) with arthritis manifestation in SLE［J］.Clin Rheumatol, 2007, 26(11): 1921-1924.
	［6］李志方, 卢健红, 李景柔, 孙筱放, 廖宝平.IFN-γ基因多态性与小儿哮喘的相关性［J］.中国优生与遗传杂志, 2006, 14(9):22-23.
	［7］Gentile DA, Doyle WJ, Zeevi A, Howe-Adams J, Kapadia S, Trecki J, et al. Cytokine gene polymorphisms moderate illness severity in infants with respiratory syncytial virus infection［J］. Hum Immunol, 2003, 64(3): 338-344.
	［8］Tal A, Bavilski C, Yohai D, Bearman JE, Gorodisher R, Moses SW, et al. Dexamethasone and salbutamol in the treatment of acute wheezhing in infants［J］. Pediatrics, 1983, 71(1):13-18.
	［9］Aberle JH, Aberle SW, Rebhandl W, Pracher E, Kundi M, Popow-Kraupp T. Decreased interferon-gamma response in respiratory syncytial virus compared to other respiratory viral infections in infants［J］. Clin Exp Immunol, 2004, 137(1): 146-150.
	［10］Lee FE, Walsh EE, Falsey AR, Lumb ME, Okam NV, Liu N, et al. Human infant respiratory syncytial virus (RSV)-specific type 1 and 2 cytokine responses ex vivo during primary RSV infection［J］. J Infect Dis, 2007, 195(12): 1779-1788.
	［11］Semple MG, Dankert HM, Ebrahimi B, Correia JB, Booth JA, Stewart JP, et al. Severe respiratory syncytial virus bronchiolitis in infants is associated with reduced airway interferon gamma and substance P［J］. PLoS ONE, 2007, 2 (10): e1038.
	［12］Lee YM, Miyahara N, Takeda K, Prpich J, Oh A, Balhorn A, et al. IFN-gamma production during initial infection determines the outcome of reinfection with respiratory syncytial virus［J］. Am J Respir Crit Care Med, 2008, 177(2): 208-218.
	［13］李兰，王智斌，李敏，张剑波，陈昌辉，李波，等.呼吸道合胞病毒毛细支气管炎患儿T细胞亚群检测的临床价值［J］.中国当代儿科杂志，2005，7（5）：421-422.
	［14］张宝琴，宋晓红，宋红霞，罗树舫，雷春莲.干扰素、白介素4在小儿肺炎支原体肺炎合胞病毒性肺炎发病中的作用［J］.中国当代儿科杂志，2002，4（2）：143-144.
	［15］张艳敏，雷春莲，成革胜，杨玉琮.RSV感染患儿外周血CD4、CD5、RO+、CD45 RA+表达变化的研究［J］.中国当代儿科杂志，2001, 3（3）：260-261.
	［16］朱启镕, 俞慧, 顾绍庆, 王建设，葛艳玲，谢新宝.干扰素γ和肿瘤坏死因子α 基因单核苷酸多态性与宫内乙肝病毒感染易感性研究［J］.临床儿科杂志, 2005, 23(7):446-449.
	［17］张平安, 吴健民, 李艳.干扰素γ基因内含子1+874位点多态性与乙型肝炎病毒感染关系的研究［J］.中华流行病学杂志, 2006, 27(1):41-43.
	［18］Chen X, Xu J, Chen Z, Feng X, Zhou Y, Ren Q, et al. Interferon-gamma +874A/T and interleukin-4 intron3 VNTR gene polymorphisms in Chinese patients with idiopathic thrombocytopenic purpura［J］. Eur J Haematol, 2007, 79(3):191-197.
	［19］Snyder LD, Hartwig MG, Ganous T, Davis RD, Herczyk WF, Reinsmoen NL, et al. Cytokine gene polymorphisms are not associated with bronchiolitis obliterans syndrome or survival after lung transplant ［J］.J Heart Lung Transplant, 2006, 25(11):1330-1335.