Abstract Objective To study the expression level of plasma miR-106b-5p in primary immune thrombocytopenia (ITP) and its correlation with the levels of T helper 17 cell (Th17) and regulatory T cell (Treg) and the Th17/Treg ratio. Methods A total of 79 children with ITP (ITP group) and 40 healthy children (control group) were selected as subjects. According to the treatment response, the 79 children with ITP were divided into three groups: complete response (n=40), partial response (n=18), and non-response (n=21). Quantitative real-time PCR was used to measure the expression level of miR-106b-5p. Flow cytometry was used to measure the frequencies of Th17 and Treg, and the Th17/Treg ratio was calculated. The correlation of the expression level of plasma miR-106b-5p with the frequencies of Th17 and Treg and the Th17/Treg ratio was analyzed. Results Compared with the control group, the ITP group had significantly higher levels of miR-106b-5p, Th17, and Th17/Treg ratio (P<0.05) and a significantly lower level of Treg (P<0.05). After treatment, the ITP group had significant reductions in the levels of miR-106b-5p, Th17, and Th17/Treg ratio (P<0.05) and a significant increase in the level of Treg (P<0.05). Compared with the partial response and non-response groups, the complete response group had significantly lower levels of miR-106b-5p, Th17, and Th17/Treg ratio (P<0.05) and a significantly higher level of Treg (P<0.05). The correlation analysis showed that in the children with ITP, the expression level of plasma miR-106b-5p was positively correlated with the Th17 level and the Th17/Treg ratio (r=0.730 and 0.816 respectively; P<0.001) and was negatively correlated with the Treg level (r=-0.774, P<0.001). Conclusions A higher expression level of miR-106b-5p and Th17/Treg imbalance may be observed in children with ITP. The measurement of miR-106b-5p, Th17, Treg, and Th17/Treg ratio during treatment may be useful to the evaluation of treatment outcome in children with ITP.
WANG Wen-Fang,WANG Xu-Song,TAN San-Yang et al. Expression of miR-106b-5p in children with primary immune thrombocytopenia and its correlation with T cells[J]. CJCP, 2022, 24(4): 411-416.
WANG Wen-Fang,WANG Xu-Song,TAN San-Yang et al. Expression of miR-106b-5p in children with primary immune thrombocytopenia and its correlation with T cells[J]. CJCP, 2022, 24(4): 411-416.
Elnaenaey WA, Omar OM, Aboelwafa RA. Increased expression of IL-17A and IL-17F is correlated with RUNX1 and RORγT in pediatric patients with primary immune thrombocytopenia[J]. J Pediatr Hematol Oncol, 2021, 43(3): e320-e327. PMID: 33633027. DOI: 10.1097/MPH.0000000000002108.
Gu H, Chen ZP, Shi XD, et al. Increased proportion of Th17/Treg cells at the new diagnosed stage of chronic immune thrombocytopenia in pediatrics: the pilot study from a multi-center[J]. Eur J Pediatr, 2021, 180(11): 3411-3417. PMID: 34046719. DOI: 10.1007/s00431-021-04121-z.
Li QZ, Liu Y, Wang XJ, et al. Regulation of Th1/Th2 and Th17/Treg by pDC/mDC imbalance in primary immune thrombocytopenia[J]. Exp Biol Med (Maywood), 2021, 246(15): 1688-1697. PMID: 33938255. DOI: 10.1177/15353702211009787.
Wang SC, Yang KD, Lin CY, et al. Intravenous immunoglobulin therapy enhances suppressive regulatory T cells and decreases innate lymphoid cells in children with immune thrombocytopenia[J]. Pediatr Blood Cancer, 2020, 67(2): e28075. PMID: 31736241. DOI: 10.1002/pbc.28075.
Atabaki M, Shariati-Sarabi Z, Barati M, et al. MicroRNAs as the important regulators of T helper 17 cells: a narrative review[J]. Iran J Allergy Asthma Immunol, 2020, 19(6): 589-601. PMID: 33463128. DOI: 10.18502/ijaai.v19i6.4928.
Sun YQ, Hou Y, Meng GY, et al. Proteomic analysis and microRNA expression profiling of plasma-derived exosomes in primary immune thrombocytopenia[J]. Br J Haematol, 2021, 194(6): 1045-1052. PMID: 34337736. DOI: 10.1111/bjh.17720.
Hua MQ, Li J, Wang CY, et al. Aberrant expression of microRNA in CD4+ cells contributes to Th17/Treg imbalance in primary immune thrombocytopenia[J]. Thromb Res, 2019, 177: 70-78. PMID: 30856381. DOI: 10.1016/j.thromres.2019.03.005.
Eroglu F, Dokur M, Ulu Y. MicroRNA profile in immune response of alveolar and cystic echinococcosis patients[J]. Parasite Immunol, 2021, 43(7): e12817. PMID: 33410199. DOI: 10.1111/pim.12817.
Qian C, Yan WY, Li TD, et al. Differential expression of miR-106b-5p and miR-200c-3p in newly diagnosed versus chronic primary immune thrombocytopenia patients based on systematic analysis[J]. Cell Physiol Biochem, 2018, 45(1): 301-318. PMID: 29402802. DOI: 10.1159/000486811.
He Y, Ji DX, Lu W, et al. Bone marrow mesenchymal stem cell-derived exosomes induce the Th17/Treg imbalance in immune thrombocytopenia through miR-146a-5p/IRAK1 axis[J]. Hum Cell, 2021, 34(5): 1360-1374. PMID: 34052997. DOI: 10.1007/s13577-021-00547-7.
Jafarzadeh A, Marzban H, Nemati M, et al. Dysregulated expression of miRNAs in immune thrombocytopenia[J]. Epigenomics, 2021, 13(16): 1315-1325. PMID: 34498489. DOI: 10.2217/epi-2021-0092.
Cheng LY, Liu CJ, Li F, et al. The prediction value of Treg cell subtype alterations for glucocorticoid treatment in newly diagnosed primary immune thrombocytopenia patients[J]. Thromb Res, 2019, 181: 10-16. PMID: 31323447. DOI: 10.1016/j.thromres.2019.07.001.
