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Expression of vascular endothelial growth factor is related to microvessel injury of renal interstitium in children with Henoch Sch nlein purpura nephritis
ZENG Xue-Qi, XU Zi-Chuan, DANG Xi-Qiang, HE Xiao-Jie, YI Zhu-Wen
Chinese Journal of Contemporary Pediatrics ›› 2009, Vol. 11 ›› Issue (9) : 717-721.
PDF(1377 KB)
PDF(1377 KB)
Expression of vascular endothelial growth factor is related to microvessel injury of renal interstitium in children with Henoch Sch nlein purpura nephritis
OBJECTIVE: To investigate the relationship between vascular endothelial growth factor (VEGF) expression and microvessel injury of renal interstitium in children with Henoch-Sch?nlein purpura nephritis (HSPN). METHODS: Thirty-two children with HSPN and who had not received glucocorticoid or immunodepressants treatment before hospitalization were enrolled. Five children undergoing nephrectomy due to renal trauma were used as the control group. Renal samples were stained by hematoxylin and eosin and renal pathological changes were evaluated semiquantitatively. CD34 and VEGF expression was detected by immunohistochemistry. CD34 was used as the marker for endothelial cells of renal microvessels. The microvessel density (MVD) was calculated by CD34 immunostaining. RESULTS: Compared with the control and the renal pathological grade II HSPN groups, MVD in the grade III and above HSPN groups decreased significantly, with an obvious reduction in MVD with the increased renal pathological grade (P<0.05). The renal microvessel score in the grades IIIa, IIIb, IV, and V HSPN groups decreased obviously compared with that in the control group. The renal microvessel score decreased with the increased renal pathological grade (P<0.05). VEGF expression in the grade II HSPN group was higher (P<0.05), while that in the grades IV and V HSPN group was lower than that in the control group (P<0.05). VEGF expression in the HSPN group showed a significant reduction with the increased renal pathological grade (P<0.05). There was a positive correlation between VEGF expression and MVD in renal tissue in the HSPN group (r=0.935, P<0.01). CONCLUSIONS: The decreased expression of VEGF may be responsible for the renal pathological damage and microvessel injury in HSPN.［Chin J Contemp Pediatr, 2009, 11 (9):717-721］
Henoch-Sch?nlein purpura nephritis / Vascular endothelial growth factor / Microvessel injury / Child
［1］许自川，曾雪琪, 党西强, 何小解.Th1/Th2迁移与紫癜性肾炎的研究进展［J］. 国际病理科学与临床杂志, 2007, 27(1):64-67.
［2］Ohashi R, Shimizu A, Masuda Y, Kitamura H, Ishizaki M, Sugisaki Y, et al. Peritubular capillary regression during the progression of experimental obstructive nephropathy［J］.Am Soc Nephrol, 2002, 13(7):1795-1805.
［3］Kang DH, Kangellis J, Hugo C, Truong L, Anderson S, Kerjaschki D, et al. Role of The microvascular endothelium in progressive renal disease［J］. J Am Soc Nephrol, 2002, 13(3):806-816.
［4］Kang DH, Anderson S, Kim YG, Mazzalli M, Suga S, Jefferson JA, et al. Impaired angiogenesis in the aging kidney: vascular endothelial growth factor and thrombospondin-1 in renal disease［J］. Am J Kidney Dis, 2001, 37(3):601-611
［5］Ohashi R, Kitamura H, Yamanaka N. Peritubular capillary injury during the progression of experimental glomerulonephritis in rats［J］. J Am Soc Nephrol, 2000, 11(1):47-56.
［6］Ferrara N, Gerber HO, LeCouter J. The biology of VEGF and its receptors［J］.Nat Med, 2003, 9(6):669-676.
［7］Gera N, Tzafra C, Stela G, Zoya P. Vascular endothelial growth factor (VEGF) and its receptors［J］. FASEB J, 1999, 13(1):9-22.
［8］Schrijvers BF, Flyvbjerg A, De Vriese AS. The role of vascular endothelial growth factor(VEGF) in renal pathophysiology［J］. Kidney Int, 2004, 65(6):2003-2017.
［9］张建江, 易著文，何小解, 杨华彬, 丁娟娟, 莫双红. 紫癜性肾炎患儿肾间质血管损害与临床的关系［J］.实用儿科临床杂志, 2006, 21(8):487-489.
［10］中华医学会儿科学分会肾脏病学组.小儿肾小球疾病的临床分类、诊断及治疗［J］.中华儿科杂志, 2001, 39(12):746-749.
［11］Weidner N. Tumoural vascularity as a prognostic in cancer patients: the evidence continues to grow［J］. J Pathol, 1998, 184（2）：119-122
［12］吴杰, 陈香美,师锁柱. 血管病变在1 005例IgA肾病中的意义及其影响因素［J］.中华医学杂志, 2003, 83(4):289-293.
［13］Ohashi R, Shimizu A, Masuda Y, Hiroshi K, Masamichi I, Yuichi S, et al. Peritubular capillary regression during the progression of experimental obstructive nephropathy［J］. J Am Soc Nephrol, 2002, 13(7):1795-1805.
［14］Bailey E, Bottomley MJ, Westwell S, Pringle JH, Furness PN, Feehally J, et al. Vascular endothelial growth factor mRNA expression in minimal change, membranous, and diabetic nephropathy demonstrated by non-isotopic in situ hybridization［J］.J Clin Pathol, 1999, 52(10):735-738.
［15］Topaloglu R, Sungur A, Baskin E, Besbab N, Saatci U, Bakkaloglu A, et al. Vascular endothelial growth factor in Henoch-Schonlein purpura［J］. J Rheumatol, 2001, 28(10):2269-2273.
［16］张秋业, 刘伟, 雷珂, 董增义. 过敏性紫癜患儿血浆可溶性细胞间粘附分子1和血管内皮生长因子水平的变化［J］.中华儿科杂志, 2004, 42(6):454-455.
［17］Simon M, Rockl W, Horning C, Grone EF, Theis H, Weich HA, et al. Receptors of vascular endothelial growth factor/vascular permeability factor(VEGF/VPF) in fetal and adult human kidney:localization and VEGF binding site［J］. Am Soc Nephrol, 1998, 9(6):1032-1044.
［18］Kitamura H, Shimizu A, Masuda Y, Kitamura H,Shimizu A, Masuda Y, et al. Apoptosis in glomerular endothelial cells during the development of glomerulosclerosis in the remnant-kidney model［J］.Exp Nephrol, 1998, 6(4):328-336.
［19］Shimizu A, Kitamura H, Masuda Y, Ishizaki M, Sugisaki Y, Yamanaka N. Rare glomerular capillary regeneration and subsequent capillary regression with endothelial cell apoptosis in progressive glomerulonephritis［J］. Am J Pathol, 1997, 151(5):1231-1239.
［20］张国珍，吴小川，易红，彭晓杰，党西强，何小解，等.儿童紫癜性肾炎临床与病理相关性分析［J］.中国当代儿科杂志，2007， 9（2）：129-132.
［21］章高平，党西强，易著文，何小解，张建江，吴小川，等.肥大细胞在过敏性紫癜肾炎患儿肾间质纤维化中的作用［J］.中国当代儿科杂志，2007，9（2）：125-128.
［22］Kang DH, Hughes J, Mazzali M, George FS, Richard JJ. Impaired angiogenesis in the remnant kidney model: II. Vascular endothelial growth factor administration reduces renal fibrosis and stabilizes renal function［J］.J Am Soc Nephrol, 2001, 12(7):1448-1457.
