Role of caspase-8 in TRAIL-induced apoptosis of neuroblastoma cell lines

TONG Hai-Xia; LU Chun-Wei; WANG Li-Wei; WANG Qiu-Shi; ZHANG Ji-Hong

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Chinese Journal of Contemporary Pediatrics ›› 2010, Vol. 12 ›› Issue (11) : 902-907.

EXPERIMENTAL RESEARCH

Role of caspase-8 in TRAIL-induced apoptosis of neuroblastoma cell lines

TONG Hai-Xia, LU Chun-Wei, WANG Li-Wei, WANG Qiu-Shi, ZHANG Ji-Hong

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Abstract

OBJECTIVE: The aim of this study was to investigate whether the induction of caspase-8 by γ-interferon (IFNγ) renders neuroblastoma (NB) cells sensitive to tumor necrosis factor related apoptosis inducing ligand(TRAIL). METHODS: Caspase-8 mRNA expression was determined by RT-PCR. The effects of IFNγ, TRAIL, IFNγ +TRAIL and caspase-8 inhibitor+ TRAIL on the growth and apoptosis of NB cells were detected with the methods of reduction rate of Alamar Blue assay and flow cytometry. The relative caspase-8 activity was measured with colorimetric assay. RESULTS: Caspase-8 expression was detectable in CHP212 cells which were sensitive to TRAIL, with an increased expression after treatment with IFNγ. Caspase-8 was undetectable in SH-SY5Y(SY5Y) cells which were resistant to TRAIL, but an increased expression of caspase-8 mRNA was found after treatment with IFNγ. Moreover, TRAIL combined with IFNγ induced apoptosis in SY5Y cells. The relative caspase-8 activity of CHP212 cells increased with the prolonged TRAIL action time. The relative caspase-8 activity of SY5Y cells in the IFNγ+TRAIL group was significantly higher than those of the control, IFNγ, TRAIL and inhibitor groups. CONCLUSIONS: NB cells expressing caspase-8 are sensitive to TRAIL. TRAIL induces apoptosis in NB cells with an increase of relative caspase-8 activity.［Chin J Contemp Pediatr, 2010, 12 (11):902-907］

Key words

Neuroblastoma / Caspase-8 / TRAIL / γ-interferon / Cell line

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TONG Hai-Xia, LU Chun-Wei, WANG Li-Wei, WANG Qiu-Shi, ZHANG Ji-Hong. Role of caspase-8 in TRAIL-induced apoptosis of neuroblastoma cell lines[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(11): 902-907

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