Abstract OBJECTIVE: To study the expression of insulin receptor substrate-1(IRS-1) and insulin receptor substrate-2 (IRS-2) in pancreas of rats with intrauterine growth retardation (IUGR). METHODS: An IUGR rat model was prepared by protein malnutrition during pregnancy. The pancreas samples of the IUGR pups were obtained at birth, and 3 weeks and 8 weeks of age. The expression of IRS-1 and IRS-2 mRNA were ascertained by RT-PCR. Western blot was used to measure the protein expression of IRS-1 and IRS-2. The rat pups born from the mother rats who received normal diet during pregnancy severed as the control group. RESULTS: The expression levels of IRS-2 mRNA and protein in pancreas of the IUGR group were significantly lower than those in the control group at all three time points (P<0.05). There were no significant differences in the expression levels of IRS-1 mRNA and protein in pancreas between the IUGR and the control groups. CONCLUSIONS: The IRS-2 expression levels in pancreas in IUGR rats decrease significantly at birth, and 3 weeks and 8 weeks of age. This might be one of the molecular mechanisms for the development of metabolic syndrome in later life in IUGR individuals.［Chin J Contemp Pediatr, 2010, 12 (12):972-975］
LI Yao,XIN Ying. Expression of insulin receptor substrates in pancreas of rats with intrauterine growth retardation[J]. 中国当代儿科杂志, 2010, 12(12): 972-975.
LI Yao,XIN Ying. Expression of insulin receptor substrates in pancreas of rats with intrauterine growth retardation[J]. CJCP, 2010, 12(12): 972-975.
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