Levels of nerve growth factor and interleukin-4 in the induced sputum of children with cough variant asthma

LI Hong-Zi, JIN Zheng-Yong, YUAN Xi-Zhe, JIN Chun-Ji

Chinese Journal of Contemporary Pediatrics ›› 2012, Vol. 14 ›› Issue (12) : 924-927.

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Chinese Journal of Contemporary Pediatrics ›› 2012, Vol. 14 ›› Issue (12) : 924-927.
CLINICAL RESEARCH

Levels of nerve growth factor and interleukin-4 in the induced sputum of children with cough variant asthma

  • LI Hong-Zi, JIN Zheng-Yong, YUAN Xi-Zhe, JIN Chun-Ji
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Abstract

OBJECTIVE: To examine the levels of nerve growth factor (NGF) and interleukin-4 (IL-4) in the induced sputum of children with cough variant asthma (CVA), with the aim of studying the roles of NGF and IL-4 in childhood CVA. METHODS: Thirty-four children with CVA were enrolled in this study. Twenty healthy children were used as a normal control group. The induced sputum was separated into supernatant and cells. Hematoxylin and eosin staining was used to count differential cells. The expression of NGF and IL-4 in supernatant was measured using ELISA. The mRNA expression of NGF and IL-4 in cells was determined by Real-time PCR analysis. RESULTS: The percentage of eosinophils in the CVA group was significantly higher than in the control group [(13.4±3.6)% vs (2.6±1.7)%; P<0.01]. The expression of NGF and IL-4 protein and mRNA in induced sputum was significantly higher in the CVA group than in the control group (P<0.05). The expression of NGF and IL-4 protein and mRNA was positively correlated with the percentage of eosinophils (P<0.01). The expression of NGF and IL-4 protein and mRNA in induced sputum was significantly reduced in the CVA group after treatment (P<0.05). CONCLUSIONS: Eosinophils infiltration and increased expression of NGF and IL-4 play key roles in the development of childhood CVA, suggesting that they may be useful in the diagnosis and treatment of childhood CVA.

Key words

Cough variant asthma / Nerve growth factor / Interleukin-4 / Child

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LI Hong-Zi, JIN Zheng-Yong, YUAN Xi-Zhe, JIN Chun-Ji. Levels of nerve growth factor and interleukin-4 in the induced sputum of children with cough variant asthma[J]. Chinese Journal of Contemporary Pediatrics. 2012, 14(12): 924-927

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