OBJECTIVE: To understand if human parvovirus B19 (HPV B19) is one of causes of respiratory infection in children. METHODS: HPV B19 DNA was detected by the nested PCR technique in serum and pharyngeal secretions of 256 children with respiratory infection between September, 2000 and August, 2001. One hundred and four normal children without respiratory infection within 2-4 months were served as the control group.RESULTS: The total positive rate of HPV B19 DNA in serum and pharyngeal secretions in the observed group was significantly higher than that of the control group ( 18.8% vs 5.8% and 20.1% vs 3.9% , respectively; both P< 0.01 ). Of the 256 cases, there were 15, 18, 7 and 8 cases of positive HPV B19 DNA in serum, and there were 17, 20, 8 and 9 cases in pharyngeal secretions in the first, second, third and fourth quarters respectively. The positive rate of HPV B19 DNA was significantly higher both in serum and pharyngeal secretions in the first and second quarters than that of the third and fourth quarters (P< 0.01 ). CONCLUSIONS: HPVB19 is one of the causative agents for respiratory infection in children. The incidence of respiratory infection with HPVB19 in the first and second quarters is higher than that of the third and fourth quarters"/>
Detection of Human Parvovirus B19 in Children with Respiratory Infection
Abstract OBJECTIVE: To understand if human parvovirus B19 (HPV B19) is one of causes of respiratory infection in children. METHODS: HPV B19 DNA was detected by the nested PCR technique in serum and pharyngeal secretions of 256 children with respiratory infection between September, 2000 and August, 2001. One hundred and four normal children without respiratory infection within 2-4 months were served as the control group.RESULTS: The total positive rate of HPV B19 DNA in serum and pharyngeal secretions in the observed group was significantly higher than that of the control group ( 18.8% vs 5.8% and 20.1% vs 3.9% , respectively; both P< 0.01 ). Of the 256 cases, there were 15, 18, 7 and 8 cases of positive HPV B19 DNA in serum, and there were 17, 20, 8 and 9 cases in pharyngeal secretions in the first, second, third and fourth quarters respectively. The positive rate of HPV B19 DNA was significantly higher both in serum and pharyngeal secretions in the first and second quarters than that of the third and fourth quarters (P< 0.01 ). CONCLUSIONS: HPVB19 is one of the causative agents for respiratory infection in children. The incidence of respiratory infection with HPVB19 in the first and second quarters is higher than that of the third and fourth quarters
