OBJECTIVE: To explore whether the transcription factor NF-κB takes part in baicalin-induced differentiation of bone marrow stromal cells (MSCs) into neurons. METHODS: MSCs from adult rats were induced by baicalin in the serum-free medium for 6 hs. The un-treated cells, as the control group, were only induced in the serum-free medium without baicalin. The expression of neuronal specific markers was evaluated by indirect immunofluorescence cytochemistry staining. The activity of NF-κB was measured by the presence of the NF-κB subunit RelA ( p65) translocated into the nucleus in the same way. RESULTS: After the induction by baicalin, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and extensive networks of processes, and the expression of neuron-specific markers was detectable 6 ds after the induction. Neuronal specific marker proteins did not express in the control group. Six days after the induction, the P65 positive rate in the cytoplasm of the control group decreased to (18.4 ± 3.0) % , while in the baicalin group, the P65 positive rate in the cytoplasm was (84.8 ± 3.0)%. CONCLUSIONS: Baicalin may inhibit the activation of NF-κB, which may act in the differentiation of MSCs into neurons. [Chin J Contemp Pediatr, 2003, 5(1): 1 - 4]"/>
Role of NF-κB in Baicalin Induced Differentiation of Rat Bone Marrow Stromal Cells into Neurons
Abstract OBJECTIVE: To explore whether the transcription factor NF-κB takes part in baicalin-induced differentiation of bone marrow stromal cells (MSCs) into neurons. METHODS: MSCs from adult rats were induced by baicalin in the serum-free medium for 6 hs. The un-treated cells, as the control group, were only induced in the serum-free medium without baicalin. The expression of neuronal specific markers was evaluated by indirect immunofluorescence cytochemistry staining. The activity of NF-κB was measured by the presence of the NF-κB subunit RelA ( p65) translocated into the nucleus in the same way. RESULTS: After the induction by baicalin, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and extensive networks of processes, and the expression of neuron-specific markers was detectable 6 ds after the induction. Neuronal specific marker proteins did not express in the control group. Six days after the induction, the P65 positive rate in the cytoplasm of the control group decreased to (18.4 ± 3.0) % , while in the baicalin group, the P65 positive rate in the cytoplasm was (84.8 ± 3.0)%. CONCLUSIONS: Baicalin may inhibit the activation of NF-κB, which may act in the differentiation of MSCs into neurons. [Chin J Contemp Pediatr, 2003, 5(1): 1 - 4]
JIA Yan-Jie,YANG Yu-Jia,SONG Jian-Hui et al. Role of NF-κB in Baicalin Induced Differentiation of Rat Bone Marrow Stromal Cells into Neurons[J]. 中国当代儿科杂志, 2003, 5(1): 1-4.
JIA Yan-Jie,YANG Yu-Jia,SONG Jian-Hui et al. Role of NF-κB in Baicalin Induced Differentiation of Rat Bone Marrow Stromal Cells into Neurons[J]. CJCP, 2003, 5(1): 1-4.
