OBJECTIVE:To explore the NS gene structure and mutation of Chinese strains of human Parvovirus B19 (HPV B19). METHODS: NS genes of R19-XA17, XA18 and XA20 strains from three Chinese children with acute aplastic anemia were amplified by PCR, and then the amplified products were sequenced directly. RESULTS: Compared with the sequence of Au strain, four bases of NS genes of B19-XA17, XA18 and XA20 strains changed and their coding amino acids changed also. CONCLUSIONS: Genetic diversity exists in mucleotide sequences of NS gene of HPV B19 isolated from the serum of Chinese children. [Chin J Contemp Pediatr, 2003, 5(1): 5 - 7]"/>
Sequence Analysis and Genetic Variation of NS Gene of Human Parvovirus B19 Isolated from Chinese Children with Acute Aplastic Anemia
Abstract OBJECTIVE:To explore the NS gene structure and mutation of Chinese strains of human Parvovirus B19 (HPV B19). METHODS: NS genes of R19-XA17, XA18 and XA20 strains from three Chinese children with acute aplastic anemia were amplified by PCR, and then the amplified products were sequenced directly. RESULTS: Compared with the sequence of Au strain, four bases of NS genes of B19-XA17, XA18 and XA20 strains changed and their coding amino acids changed also. CONCLUSIONS: Genetic diversity exists in mucleotide sequences of NS gene of HPV B19 isolated from the serum of Chinese children. [Chin J Contemp Pediatr, 2003, 5(1): 5 - 7]
ZHANG Guo-Cheng,SU Guo-Ping,SUN Xin et al. Sequence Analysis and Genetic Variation of NS Gene of Human Parvovirus B19 Isolated from Chinese Children with Acute Aplastic Anemia[J]. 中国当代儿科杂志, 2003, 5(1): 5-7.
ZHANG Guo-Cheng,SU Guo-Ping,SUN Xin et al. Sequence Analysis and Genetic Variation of NS Gene of Human Parvovirus B19 Isolated from Chinese Children with Acute Aplastic Anemia[J]. CJCP, 2003, 5(1): 5-7.
