OBJECTIVE: To study the isolation and cultiv ation of neural stem cells in neonatal rats. METHODS: Cell culture, indirect immunofluo rescence cytochemistry and gene transfection techniques were used. RESULTS: The neural stem cells isolated in newborn rats had the potential to form clones, express neuroepithelial stem cell protein(nestin) and differentiate into mature neurons and astrocytes. Moreover, green fluorescent protein vector could be efficiently transfected into this cell line. CONCLUSIONS: Neural stems cells can only be characterized on a critical functional basis in terms of their undifferentiated features, capacity of self-renewal, proliferation and pluripotentiality, and can express ex vivo gene efficiently."/>
Isolation and Cultivation of Neural tem Cells in neonatal Rats
Abstract OBJECTIVE: To study the isolation and cultiv ation of neural stem cells in neonatal rats. METHODS: Cell culture, indirect immunofluo rescence cytochemistry and gene transfection techniques were used. RESULTS: The neural stem cells isolated in newborn rats had the potential to form clones, express neuroepithelial stem cell protein(nestin) and differentiate into mature neurons and astrocytes. Moreover, green fluorescent protein vector could be efficiently transfected into this cell line. CONCLUSIONS: Neural stems cells can only be characterized on a critical functional basis in terms of their undifferentiated features, capacity of self-renewal, proliferation and pluripotentiality, and can express ex vivo gene efficiently.
