Abstract OBJECTIVE: To examine the expression of macrophage migration inhibitory factor (MIF) in renal tissues obtained from children with Henoch-Sch-nlein purpura nephritis (HSPN). METHODS: The renal tissue samples were obtained from 11 children with different pathological grades of HSPN and 8 children with thin glomerular basement membrane disease (controls). The MIF expression was measured by immunohistochemistry. The correlation between MIF expression and 24 hrs urinary protein excretions was evaluated using a linear correlation analysis. RESULTS: MIF expression was seldom found in renal tissues obtained from controls. However, a significantly increased MIF expression was found and was concordant with the increased severity of renal pathology in renal tissues obtained from children with HSPN. The MIF expression in renal tissues of grade Ⅲ-Ⅳ of renal pathology was significantly higher than that in gradeⅠ-Ⅱ in children with HSPN (P<0.01). In children with HSPN, there was an increased MIF expression in renal tissues with crescent formation and inflammatory cell infiltration. Renal MIF expression was significantly positively correlated with 24 hrs urinary protein excretions in children with HSPN (P<0.01). CONCLUSIONS: MIF may play an important role in renal injury of HSPN. Up-regulation of MIF expression may reflect the degree of renal lesions in HSPN.［Chin J Contemp Pediatr, 2010, 12 (2):120-122］
ZHENG Wen-Jie,CHEN Min-Guang,CHEN Xiao-Ying et al. Renal expression of macrophage migration inhibitory factor in children with Henoch-Sch-nlein purpura nephritis[J]. 中国当代儿科杂志, 2010, 12(2): 120-122.
ZHENG Wen-Jie,CHEN Min-Guang,CHEN Xiao-Ying et al. Renal expression of macrophage migration inhibitory factor in children with Henoch-Sch-nlein purpura nephritis[J]. CJCP, 2010, 12(2): 120-122.
［1］Lan HY, Mu W, Yang N, Meinhardt A, Nikolic-Paterson DJ, Ng YY, et al. De novo renal expression of macrophage migration inhibitory factor during the development of rat crescentic glomerulonephritis［J］. Am J Pathol, 1996, 149(4):1119-1127.
［3］Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, et al. Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis［J］. Clin Exp Immunol, 2005, 139(2):338-347.
［4］Bozza M, Satoskar AR, Lin G, Lu B, Humbles AA, Gerard C, et al. Targeted disruption of migration inhibitory factor gene reveals its critical role in sepsis［J］. J Exp Med, 1999, 189(2):341-346.
［5］Lan HY, Yang N, NikolicPaterson DJ, Yu XQ, Mu W, Isbel NM, et al. Expression of macrophage migration inhibitory factor in human glomerulonephritis［J］. Kidney Int, 2000, 57(2):499-509.
［7］Yang N, Nikolic-Paterson DJ, Ng YY, Mu W, Metz C, Bacher M, et al．Reversal of established rat crescentic glomerulonephritis by blockade of macrophage migration inhibitory factor(MIF)：potential role of MIF in regulating glucocorticoid production［J］．Mol Med, 1998, 4(6)：413-424.
［8］Leung JC, Tang SC, Chan LY, Tsang AW, Lan HY, Lai KN. Polymeric IgA increases the synthesis of macrophage migration inhibitory factor by human mesangial cells in IgA nephropathy［J］. Nephrol Dial Transplant, 2003, 18(1):36-45.
［9］Tesch GH, Nikolic-Paterson DJ, Metz CN, Mu W, Bacher M, Bucala R, et al. Rat mesangial cells express macrophage migration inhibitory factor in vitro and in vivo［J］. J Am Soc Nephrol, 1998, 9(3):417-424.
［10］Jafar TH, Stark PC, Schmid CH, Landa M, Maschio G, Marcantoni C, et al. Proteinuria as a modifiable risk factor for the progression of nondiabetic renal disease［J］. Kidney Int, 2001, 60(3):1131-1140.
