Abstract OBJECTIVE: To study the expression of glucose-regulated protin 78 (GRP78) and glucose-regulated protin 94 (GRP94) in the liver tissues from children with hepatoblastoma (HB) and to investigate the possible clinicopathological values of GRP78 and GRP94 in HB. METHODS: Liver tissue specimens from 15 children with HB and 10 specimens of normal liver tissues were obtained. EnVison immunohistochemistry was used to detect the expression of GRP78 and GRP94 in the conventional paraffin-embedded liver sections. RESULTS: The positive rates of GRP78 expression (53% vs 10%; P<0.05) and GRP94 expression (60% vs 10%; P<0.05) in HB liver tissues were significantly higher than those in the normal liver tissues. The positive rates of GRP78 expression in the cases without lymphnode metastasis or in clinical stage I-II were significantly lower than those in the cases with lymphnode metastasis or in clinical stage III-IV (P<0.05). GRP94 showed a decreased tendency of positive expression in the cases without lymphnode metastasis or in clinical stage I-II when compared with the cases with lymphnode metastasis or in clinical stage III-IV, although there were no statistical differences between them. CONCLUSIONS: GRP78 and GRP94 expression might play important roles in the pathogenesis and progression of pediatric HB.［Chin J Contemp Pediatr, 2010, 12 (8):634-636］
CHEN Gan-Nong,MA Yong,YANG Zhu-Lin. Expression of GRP78 and GRP94 in the liver tissues and their clinicopathological significance in children with hepatoblastoma[J]. 中国当代儿科杂志, 2010, 12(08): 634-636.
CHEN Gan-Nong,MA Yong,YANG Zhu-Lin. Expression of GRP78 and GRP94 in the liver tissues and their clinicopathological significance in children with hepatoblastoma[J]. CJCP, 2010, 12(08): 634-636.
［3］Towu E, Kiely E, Pierro A, Spitz L . Outcome and complications after resection of hepatoblastoma ［J］. J Pediatr Surg, 2004, 39(2):199-202.
［4］Isaacs H Jr. Fetal and neonatal hepatic tumors ［J］. J Pediatr Surg, 2007, 42(11):1797-1803.
［5］Horton JD, Lee S, Brown SR , Bader J, Meier DE. Survival brends in children with hepatoblastoma ［J］. Pediatr Surg Int, 2009, 25(5):407-441.
［6］Lee AS. The glucose-regulated proteins: stress induction and clinical applications ［J］. Trends Biochem Sci, 2001, 26(8):504-510.
［7］Uramoto H, Sugio K, Oyama T, Nakata S, Ono K, Yoshimastu T, et al. Expression of endoplasmic reticulum molecular chaperone Grp78 in human lung cancer and its clinical significance ［J］. Lung Cancer, 2005, 49(1):55-62.
［8］Fernandez PM, Tabbara SO, Jacobs LK, Manning FC, Tsangaris TN, Schwartz AM, et al. Overexpression of the glucose-regulated stress gene GRP78 in malignant but benign human breast lesions ［J］. Breast Cancer Res Treat, 2000, 59（1）：15-26.
［9］Langer R, Feith M, Siewert JR, Wester HJ, Hoefler H. Expression and clinical significance of glucose-regulated proteins GRP78(BiP) and GRP94 (GP96) in human adenocarcinomas of the esophagus ［J］. BMC Cancer, 2008, 8:70-75.
［10］Zheng HC, Takahashi H, Li XH, Hara T, Masuda S, Guan YF, et al. Overexpression of GRP78 and GRP94 are markers for aggressiver behavior and poor prognosis in gastric carcinoma ［J］. Hum Pathol, 2008, 39(7):1042-1049.
［11］Hodorova I, Rybarova S, Solar P, Vecanova J, Prokopcakova L, Bohus P, et al. GP96 and its different expression in breast carcinomas ［J］. Neoplasma, 2008, 55(1):31-35.
［12］Fu Y, Lee AS. Glucose regulated proteins in cancer progression, drug resistance and immunotherapy ［J］. Cancer Biol Ther, 2006, 5(7):741-744.
［13］Kubata H, Suzuki T, Lu J , Takahashi S, Sugita K, Sekiya K, et al. Increased expression of GRP94 protein is associated with decreased sensitivity to in cervical cancer cell ［J］. Int J Radiat Biol, 2005, 81(9):701-709.
［14］Nomura H, Uzawa K, Yamano Y, Fushimi K, Ishigami T, Kata Y, et al. Networkbased analysis of calcium-binding protein genes identifies Grp94 as a target in human oral carcinogenesis ［J］. Br J Cancer, 2007, 97(6):792-801.
［15］Zhang LY, Zhang XC, Wang LD, Zhang ZF, Li PL. Increased expression of GRP94 protein is associated with decreased sensitivity to adriamycin in ovarian carcinoma cell lines ［J］. Clin Exp Obstet Gynecol, 2008, 35(4):257-263.
