Culture and identification of neural stem cells from mouse embryos
ZHANG Peng-Bo, LI Wei-Song, GAO Ming, LI Ling, WANG Ni, LEI Shan, LV Hai-Xia , CHEN Xin-Lin, LIU Yong
Department of Anesthesiology, Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, China. Email: zhpbo@163.com
Abstract OBJECTIVE: The purpose of this study was to culture and identify neural stem cells from mouse embryos in vitro using a modified method and provide a basis for further study of the biology of neural stem cells under hypoxia. METHODS: The cells were isolated mechanically from the front cortex of fetal Institute of Cancer Research (ICR) mice on embryonic day 14. They were passaged by mechanical dissociation and enzymatic digestion. The neurospheres were identified by immunofluorescent staining of nestin. Cell differentiation was induced by 1% fetal bovine serum and then the cells were identified by immunohistochemistry of β-tubulin III and GFAP. RESULTS: The cells obtained from the front cortex of fetal ICR mice had the capacity of forming neurospheres which showed nestin immunoreactive positivity. After being induced by 1% fetal bovine serum, the cells were differentiated into β-tubulin III-positive cells and GFAP-positive cells. CONCLUSIONS: Using mechanical dissociation of primary cells and mechanical dissociation with enzymatic digestion of primary cells, the NSCs from the front cortex of mouse embryos can be obtained.
Key words ：
Neural stem cells
Embryo
Cell culture
Mice
Cite this article:
ZHANG Peng-Bo,LI Wei-Song,GAO Ming et al. Culture and identification of neural stem cells from mouse embryos[J]. 中国当代儿科杂志, 2011, 13(3): 244-247.
ZHANG Peng-Bo,LI Wei-Song,GAO Ming et al. Culture and identification of neural stem cells from mouse embryos[J]. CJCP, 2011, 13(3): 244-247.
URL:
http://www.zgddek.com/EN/ OR http://www.zgddek.com/EN/Y2011/V13/I3/244
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