Abstract OBJECTIVE: To study the expression of CD4+ CD25int/high CD127low regulatory T cells in peripheral blood (PB) and its relation to the quantity of Hb, WBC and platelet (Plt) in children with aplastic anemia (AA). METHODS: Expression of CD4+ CD25int/high CD127low in PB was detected by flow cytometry in 22 children with AA before and after treatment and in 15 healthy controls. The relationships between CD4+CD25highCD127low and the quantity of Hb, WBC and Plt were evaluated. RESULTS: Compared to controls, the percentages of CD4+ CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ in PB of AA patients decreased markedly at the active phase (P﹤0.05) . By the recovery phase, the percentages of CD4+CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ increased significantly to the levels similar to the controls. There were significant positive relationships between the expression of CD4+CD25highCD127low cells and the quantity of Hb, WBC and Plt (r=0.499, 0.526, 0.540 respectively; P﹤0.05). CONCLUSIONS: The decrease of the percentage of CD4+CD25int/highCD127low regulatory T cells might be associated with the development of pediatric AA. The CD4+CD25int/highCD127low regulatory T cells can serve as a marker for the evaluation of disease severity as well as a target of further study on immune treatment of AA.
WANG Xi-Ge,CAO Yi-Ming,WANG Xiao-Ge. Detection and significance of CD4+ CD25int/high CD127low regulatory T cells in pediatric aplastic anemia[J]. 中国当代儿科杂志, 2011, 13(4): 292-295.
WANG Xi-Ge,CAO Yi-Ming,WANG Xiao-Ge. Detection and significance of CD4+ CD25int/high CD127low regulatory T cells in pediatric aplastic anemia[J]. CJCP, 2011, 13(4): 292-295.
［4］Lutsiak ME, Semnani RT, De Pascalis R, Kashmiri SV, Schlom J, Sabzevari H. Inhibition of CD4+25+ T regulatory cell function implicated in enhanced immune response by low-dose cyclophosphamide［J］.Blood, 2005, 105(7): 2862-2868.
［5］Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, et al. Cord blood CD4+CD25+ -derived T regulatory cells lines express FOXP3 protein and manifest potent suppressor function［J］.Blood, 2005, 105(2):750-758.
［6］Toda A, Piccirillo CA. Development and function of naturally occurring CD4+CD25+ regulatory T cells［J］.J Leukoc Biol, 2006, 80(3): 458-470.
［7］Kamikozuru K, Fukunaga K, Hirota S, Hida N, Ohda Y, Yoshida K, et al. The expression profile of functional regulatory T cells,CD4+CD25high+/forkhead box protein P3+, in patients with ulcerative colitis during active and quiescent disease［J］. Clin Exp Immunol, 2009, 156(2): 320-327.
［10］Hartigan-O'Connor DJ, Poon C, Sinclair E, McCune JM. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127),allowing consistent identification and sorting of live cells［J］. J Immunal Methods, 2007, 319(1-2): 41-52.
［12］Moniuszko M, Bodzenta-Lukaszyk A, Dabrowska M. Effects of oral glucocorticoid therapy on CD4+CD25+CD127- and CD4+CD25high T cell levels in asthmatic patients［J］. Inflammation, 2010, 33(6): 415-420.
