Abstract OBJECTIVE: To investigate the changes of CD4+CD25+Foxp3+ regulatory T cells (Treg) and T helper cells (Th1/Th2) in peripheral blood and their roles in the severity evaluation in children with asthma. METHODS: One hundred and fifty children with asthma were classified into acute attack (94 cases) and remission (56 cases) groups according to their clinical features, and the acute attack children were subdivided into mild asthma (54 cases) and severe asthma (40 cases) groups. Fifty healthy children were enrolled as a control group. The levels of CD4+CD25+Foxp3+ Treg, CD4+IFN-γ+ Th1 and CD4+IL-4+ Th2 in peripheral blood were measured by flow cytometer. RESULTS: The mean levels of CD4+CD25+Foxp3+ Treg and the ratio of Th1/Th2 in asthmatic children were lower than those in the control group (P<0.01). The Treg levels and the ratio of Th1/Th2 in the acute attack group were lower than those in the remission group and in the control group (P<0.01). The Treg levels in the severe asthma group were lower than those in the mild asthma group (P<0.01). There was a remarkably negative correlation between Treg levels and the asthma severity (r=-0.737, P<0.01), and the Th1/Th2 ratio was also negatively correlated with the asthma severity (r=-0.615, P<0.01). The Treg levels were positively correlated with the Th1/Th2 ratio (r=0.856, P<0.01). CONCLUSIONS: The Treg levels decrease remarkably and Th subsets imbalance occurs in children with asthma. This suggests that Treg and Th immunity play important roles in the pathogenesis of asthma. The Treg levels and the ratio of Th1/Th2 in peripheral blood may be useful in the evaluation of severity in children with asthma.
YANG Yan-Li,PAN Yu-Qin,HE Bang-Shun et al. Changes of regulatory T cells and T helper cells in peripheral blood and their roles in the severity evaluation in children with asthma[J]. 中国当代儿科杂志, 2011, 13(6): 482-486.
YANG Yan-Li,PAN Yu-Qin,HE Bang-Shun et al. Changes of regulatory T cells and T helper cells in peripheral blood and their roles in the severity evaluation in children with asthma[J]. CJCP, 2011, 13(6): 482-486.
［2］Smit JJ, Boon L, Lukacs NW. Respiratory virus-induced regulation of asthma-like responses in mice depends upon CD8 T cells and interferon-gamma production［J］. Am J Pathol, 2007, 171(6): 1944-1951.
［3］陈广洁. Foxp3 和 CD4+CD25+ 调节性 T 细胞研究进展［J］. 国外医学·免疫学分册, 2005, 28(1):1-4．
［4］Lin W, Truong N, Grossman WJ, Haribhai D, Williams CB, Wang J, et al. Allergic dysregulation and hyperimmunoglobulin emia E in Foxp3 mutant mice［J］. J Allergy Clin Immunol, 2005, 116(5): 1106-1115.
［5］Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells［J］. Nat Immunol, 2003, 4 (4): 330-336.
［7］Capron M, Woerly G, Kayaba H, Loiseau S, Roger N, Dombrowicz D. Invited lecture: role of membrane receptors in the release of T helper 1 and cytokines by eosinophils［J］. Int Arch Allergy Immuno, 2001, 124 (1-3): 223-226.
［8］Shi HZ, Xiao CQ, Zhong D, Qin SM, Liu Y, Liang GR,et al. Effect of inhaled Interleukin 5 on airway hyperreactivity and eosinophilia in asthmatics［J］. Am J Respir Crit Care Med, 1998, 157: 204209.
［15］Jaffar Z, Sivakuru T, Roberts K. CD4+CD25+ T cells regulate airway eosinophilic inflammation by modulating the Th2 cell phenotype［J］. J Immunol, 2004, 172(6):38423849.
［16］Taylor PA, Noelle RJ, Blazar BR. CD4+CD25+ immune regulatory cells are required for induction of tolerance to alloantigen via costimulatory blockade［J］. J Exp Med, 2001, 193(11): 1311-1318.
［17］Shi HZ, Li S, Xie ZF, Qin XJ, Qin X, Zhong XN. Regulatory CD4+CD25+ T lymphocytes in peripheral blood from patients with atopic asthma［J］. Clin Immunol, 2004, 113(2):172-178.
