Abstract OBJECTIVE: To study the levels of CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) in the peripheral blood of children with epilepsy and the roles of Tregs in the pathogenesis of epilepsy. METHODS: Forty-one children with epilepsy and thirty-eight healthy children were enrolled. The percentage of CD4+ CD25+ Foxp3+ Tregs in CD4+ T cells and the percentages of CD4+ T cells, CD8+ T cells, nature killer (NK) cells and B cells in lymphocytes were evaluated by flow cytometry. RESULTS: The percentages of peripheral blood CD4+ CD25+ Foxp3+ Tregs and CD4+ T cells and the ratio of CD4+ T cells to CD8+ T cells in epileptic children were (2.4±0.5)%, (35±5)% and 1.32±0.24 respectively, which were significantly lower than those in healthy children ［(6.1±1.2)%, (38±4)% and 1.60±0.24 respectively; P<0.05］. In contrast, the percentages of CD8+ T cells, NK cells and B cells in lymphocytes in epileptic children were significantly higher than those in healthy children［(27±3)% vs (24±3)%, (11.1±5.1)% vs (8.5±1.9)% and (24±9)% vs (16±5)% respectively; P<0.05］. CONCLUSIONS: The abnormal percentage of CD4+ CD25+ Foxp3+ Tregs in the peripheral blood may be involved in the pathogenesis of epilepsy.
［1］Marson A, Kretschmer K, Frampton GM, Jacobsen ES, Polansky JK, MacIsaac KD, et al. Foxp3 occupancy and regulation of key target genes during T-cell stimulation［J］. Nature, 2007, 445(7130): 931-935.
［2］Lehtim-ki KA, Ker-men T, Palmio J, M-kinen R, Hurme M, Honkaniemi J, et al. Increased plasma levels of cytokines after seizures in localization-related epilepsy［J］．Acta Neurol Scand, 2007, 116(4)：226-230.
［3］Sakaguchi S. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and nonself［J］. Nat Immunol, 2005, 6（4）: 345-352.
［4］Engel J. International League Against Epilepsy (ILAE). A proposed diagnostic scheme for people with epileptic seizures and with epilepsy: report of the ILAE Task Force on Classification and Terminology［J］. Epilepsia, 2001, 42(6): 796-803.
［5］EI Andaloussi A, Lesniak MS. An increase in CD4+CD25+Foxp3+ regulatory T cells in tumor-infiltrating lymphocytes of human glioblastoma multiforme［J］. Neuro Oncol, 2006, 8(3): 234-243.
［6］Bauer S, K-ller M, Cepok S, Todorova-Rudolph A, Nowak M, Nockher WA, et al. NK and CD4+ T cell changes in blood after seizures in temporal lobe epilepsy［J］. Exp Neurol, 2008, 211(2): 370-377.
［7］Montelli TC, Soares AM, Pera-oli MT. Immunologic aspects of West syndrome and evidence of plasma inhibitory effects on T cell function［J］. Arq Europsiquiatr, 2003, 61(3B): 731-737.
［9］Bhatt R, Bhatt S, Hameed M, Rameshwar P, Siegel A. Amygdaloid kindled seizures can induce functional and pathological changes in thymus of rat: role of the sympathetic nervous system［J］. Neurobiol Dis, 2006, 21（1）：127-137.
