Abstract OBJECTIVE: To measure levels of cytokines including IL-1β, IL-12, IL-18 and TNF-α in children with newly diagnosed type 1 diabetes and to analyze their correlation with clinical indices such as infection and onset time. METHODS: A total of 33 children with newly diagnosed type 1 diabetes were assigned to the case group, and 27 healthy children to the control group. The case group was further divided into increased white blood cell (WBC) and normal WBC subgroups according to peripheral WBC level. The serum levels of cytokines including IL-1β, IL-12, IL-18 and TNF-α were measured by enzyme-linked immunosorbent assay. Blood pH, blood sugar, blood lactate, fructosamine, peripheral leukocytes and neutrophils and some other clinical indices were also measured. RESULTS: The level of IL-12 in the case group was higher than in the control group (P<0.001). In the case group, the level of IL-18 was negatively correlated with onset time (r=0.413, P=0.015), the neutrophil count was positively correlated with IL-1β level (r=0.413, P=0.023) and the WBC count was positively correlated with IL-18 level (r=0.352, P=0.038). IL-1β, IL-12 and IL-18 levels in the increased WBC subgroup were higher than in the normal WBC subgroup (P<0.05 for all comparisons). CONCLUSIONS: Cytokine secretion disorders of Th1 cells exist in children with type 1 diabetes. Infections may induce cytokine secretion and might contribute to the early onset of diabetes.
LV Ling,LIU Jia-Yue,MA Jing et al. Factors associated with Th1 cytokine disorders in children with newly diagnosed type 1 diabetes[J]. CJCP, 2013, 15(1): 50-52.
LV Ling,LIU Jia-Yue,MA Jing et al. Factors associated with Th1 cytokine disorders in children with newly diagnosed type 1 diabetes[J]. CJCP, 2013, 15(1): 50-52.
［1］Knip M, Siljander H. Autoimmune mechanisms in type 1 diabetes［J］. Autoimmun Rev, 2008, 7(7): 550-557.
［2］Umeshappa CS, Huang H, Xie Y, Wei Y, Mulligan SJ, Deng Y, et al. CD4+Th-APC with acquired peptide/MHC class I and II complexes stimulate type 1 helper CD4+ and central memory CD8+T cell responses［J］. J Immunol, 2009,182(1): 193-206.
［3］Gallardo CD, Guzman MA. Immunopathology of the diabetes mellitus type 1［J］. Archivos de Alergia e Inmunologia Clinica, 2008, (39)4: 151-160.
［3］Gallardo CD, Guzman MA. Immunopathology of the diabetes mellitus type 1［J］. Archivos de Alergia e Inmunologia Clinica, 2008, (39)4: 151-160.
［4］Settin A, Ismail A, El-Magd MA, El-Baz R, Kazamel A. Gene polymorphisms of TNF-308 (G/A), IL-10(-1082) (G/A), IL-6(-174) (G/C)and IL-1Ra (VNTR) in Egyptian cases with type 1 diabetes mellitus［J］. Autoimmunity, 2009, 42(1) 50-55.
［4］Settin A, Ismail A, El-Magd MA, El-Baz R, Kazamel A. Gene polymorphisms of TNF-308 (G/A), IL-10(-1082) (G/A), IL-6(-174) (G/C)and IL-1Ra (VNTR) in Egyptian cases with type 1 diabetes mellitus［J］. Autoimmunity, 2009, 42(1) 50-55.
［5］Altinova AE, Yetkin I, Akbay E, Bukan N, Arslan M. Serum IL-18 levels in patients with type 1 diabetes: relations to metabolic control and microvascular complications［J］. Cytokine, 2008, 42(2): 217-221.
［7］Calder PC, Dimitriadis G, Newsholme P. Glucose metabolism in lymphoid and inflammatory cells and tissues［J］. Curr Opin Clin Nutr Metab Care, 2007, 10(4): 531-540.
［5］Altinova AE, Yetkin I, Akbay E, Bukan N, Arslan M. Serum IL-18 levels in patients with type 1 diabetes: relations to metabolic control and microvascular complications［J］. Cytokine, 2008, 42(2): 217-221.
［8］Wan XX, Yue SB, Peng XX, Ping Z, Gang C. Neutrophils induce the maturation of immature dendritic cells: a regulatory role of neutrophils in adaptive immune responses［J］. Immunol Invest, 2007, 36(3): 337-350.
［7］Calder PC, Dimitriadis G, Newsholme P. Glucose metabolism in lymphoid and inflammatory cells and tissues［J］. Curr Opin Clin Nutr Metab Care, 2007, 10(4): 531-540.
［8］Wan XX, Yue SB, Peng XX, Ping Z, Gang C. Neutrophils induce the maturation of immature dendritic cells: a regulatory role of neutrophils in adaptive immune responses［J］. Immunol Invest, 2007, 36(3): 337-350.
