Effect of hyperoxia on p53 and PCNA expression in fetal rat lung fibroblasts

CAI Xiao-Fang; SUN Ji-Min; LI Wen-Bin

doi:10.7499/j.issn.1008-8830.2014.09.012

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Chinese Journal of Contemporary Pediatrics ›› 2014, Vol. 16 ›› Issue (9) : 922-926. DOI: 10.7499/j.issn.1008-8830.2014.09.012

EXPERIMENTAL RESEARCH

Effect of hyperoxia on p53 and PCNA expression in fetal rat lung fibroblasts

CAI Xiao-Fang¹, SUN Ji-Min¹, LI Wen-Bin²

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Abstract

Objective To study the effect of hyperoxia exposure on the expression of p53 and proliferating cell nuclear antigen（PCNA) in fetal rat lung fibroblasts（LFs). Methods Primary rat embryonic LFs were cultured in vitro.LFs grew to subconfluence and then were randomly divided into air and hyperoxia exposure（95% O₂, 5% CO₂) groups.After LFs were cultured for 12 and 24 hours, the proliferation was analyzed by MTT. p53 mRNA level was detected by semi-quantitative reverse transcription polymerase chain reaction（RT-PCR). p53 and PCNA protein levels were determined by Western blot. Results After 12 and 24 hours of culture the growth inhibition rate of LFs was 8% and 23% respectively in the hyperoxia exposure group. p53 mRNA and protein levels increased significantly（P<0.01) in the hyperoxia exposure group after 12 and 24 hours of culture compared with the air exposure group. Hyperoxia exposure decreased PCNA expression after 24 hours of culture（P<0.01). Conclusion Hyperoxia exposure increases p53 level and decreases PCNA expression, resulting in inhibitions of LFs proliferation and DNA repair.

Key words

Hyperoxia / Lung fibroblast / p53 / Proliferating cell nuclear antigen / Rats

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CAI Xiao-Fang, SUN Ji-Min, LI Wen-Bin. Effect of hyperoxia on p53 and PCNA expression in fetal rat lung fibroblasts[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(9): 922-926 https://doi.org/10.7499/j.issn.1008-8830.2014.09.012

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