Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia

FAN Yu-Ying; YU Tao; ZHANG Jun-Mei; WANG Hua; ZHAO Gui-Feng; LIU Bo

doi:10.7499/j.issn.1008-8830.2014.09.014

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Chinese Journal of Contemporary Pediatrics ›› 2014, Vol. 16 ›› Issue (9) : 933-938. DOI: 10.7499/j.issn.1008-8830.2014.09.014

EXPERIMENTAL RESEARCH

Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia

FAN Yu-Ying¹, YU Tao¹, ZHANG Jun-Mei¹, WANG Hua¹, ZHAO Gui-Feng², LIU Bo²

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Abstract

Objective To study the changes of endogenous leukemia inhibitory factor（LIF) in neonatal rats with periventricular leukomalacia（PVL). Methods A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia（HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP. Results At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group（P<0.01). In the PVL group, the LIF protein level on the third day after HI reached a peak and was higher than the other time points（P<0.01). The change of LIF mRNA expression showed the same tendency with LIF protein. The double staining immunofluorescence showed a co-expression of LIF and GFAP. Conclusion LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.

Key words

Leukemia inhibitory factor / Periventricular leukomalacia / Hypoxia ischemia / Neonatal rats

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FAN Yu-Ying, YU Tao, ZHANG Jun-Mei, WANG Hua, ZHAO Gui-Feng, LIU Bo. Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(9): 933-938 https://doi.org/10.7499/j.issn.1008-8830.2014.09.014

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