Abstract Objective To study the changes of endogenous leukemia inhibitory factor（LIF) in neonatal rats with periventricular leukomalacia（PVL). Methods A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia（HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP. Results At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group（PPConclusion LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.
FAN Yu-Ying,YU Tao,ZHANG Jun-Mei et al. Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia[J]. CJCP, 2014, 16(9): 933-938.
FAN Yu-Ying,YU Tao,ZHANG Jun-Mei et al. Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia[J]. CJCP, 2014, 16(9): 933-938.
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