Abstract Objective To study the effects of caffeine citrate on myelin basic protein (MBP) expression in the cerebral white matter of neonatal rats with hypoxic-ischemic brain damage (HIBD) and the related mechanism.Methods Forty-eight seven-day-old Sprague-Dawley neonatal rats were randomly assigned to 3 groups:sham operation (n=16), HIBD (n=16) and HIBD+caffeine citrate (n=16). The rats in the HIBD and HIBD+caffeine citrate groups were subjected to left common carotid artery ligation, and then were exposed to 80 mL/L oxygen and 920 mL/L nitrogen for 2 hours to induce HIBD. The rats in the sham operation group were only subjected to a sham operation, without the left common carotid artery ligation or hypoxia exposure. Caffeine citrate (20 mg/kg) was injected intraperitoneally before hypoxia ischemia (HI) and immediately, 24 hours, 48 hours and 72 hours after HI. The other two groups were injected intraperitoneally with an equal volume of normal saline at the corresponding time points. On postnatal day 12, the expression of MBP in the left subcortical white matter was detected by immunohistochemistry, and the levels of adenosine A1 receptor mRNA and A2a receptor mRNA in the left brain were detected by real-time PCR.Results The expression of MBP in the left subcortical white matter in the HIBD group was lower than in the sham operation group (PPPConclusions Caffeine citrate can improve brain white matter damage following HIBD in neonatal rats and the protection mechanism might be related with the down-regulation of adenosine A1 receptor expression.
XU Fa-Lin,CHENG Hui-Qing,WANG Cai-Hong et al. Effects of caffeine citrate on myelin basic protein in neonatal rats with hypoxicischemic brain damage[J]. CJCP, 2015, 17(9): 984-988.
XU Fa-Lin,CHENG Hui-Qing,WANG Cai-Hong et al. Effects of caffeine citrate on myelin basic protein in neonatal rats with hypoxicischemic brain damage[J]. CJCP, 2015, 17(9): 984-988.
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