Effects of caffeine citrate on myelin basic protein in neonatal rats with hypoxicischemic brain damage

XU Fa-Lin; CHENG Hui-Qing; WANG Cai-Hong; ZHANG Yan-Hua; GUO Jia-Jia

doi:10.7499/j.issn.1008-8830.2015.09.020

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Chinese Journal of Contemporary Pediatrics ›› 2015, Vol. 17 ›› Issue (9) : 984-988. DOI: 10.7499/j.issn.1008-8830.2015.09.020

EXPERIMENTAL RESEARCH

Effects of caffeine citrate on myelin basic protein in neonatal rats with hypoxicischemic brain damage

XU Fa-Lin, CHENG Hui-Qing, WANG Cai-Hong, ZHANG Yan-Hua, GUO Jia-Jia

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Abstract

Objective To study the effects of caffeine citrate on myelin basic protein (MBP) expression in the cerebral white matter of neonatal rats with hypoxic-ischemic brain damage (HIBD) and the related mechanism.Methods Forty-eight seven-day-old Sprague-Dawley neonatal rats were randomly assigned to 3 groups:sham operation (n=16), HIBD (n=16) and HIBD+caffeine citrate (n=16). The rats in the HIBD and HIBD+caffeine citrate groups were subjected to left common carotid artery ligation, and then were exposed to 80 mL/L oxygen and 920 mL/L nitrogen for 2 hours to induce HIBD. The rats in the sham operation group were only subjected to a sham operation, without the left common carotid artery ligation or hypoxia exposure. Caffeine citrate (20 mg/kg) was injected intraperitoneally before hypoxia ischemia (HI) and immediately, 24 hours, 48 hours and 72 hours after HI. The other two groups were injected intraperitoneally with an equal volume of normal saline at the corresponding time points. On postnatal day 12, the expression of MBP in the left subcortical white matter was detected by immunohistochemistry, and the levels of adenosine A1 receptor mRNA and A2a receptor mRNA in the left brain were detected by real-time PCR.Results The expression of MBP in the left subcortical white matter in the HIBD group was lower than in the sham operation group (P<0.05). The MBP expression in the HIBD+caffeine citrate group was significantly higher than in the HIBD group, but was still lower than the sham operation group (P<0.05). Real-time PCR showed that the adenosine A1 receptor mRNA expression was significantly higher in the HIBD group than in the sham operation group, and it was significantly lower in the HIBD+caffeine citrate group than in the HIBD group (P<0.05).Conclusions Caffeine citrate can improve brain white matter damage following HIBD in neonatal rats and the protection mechanism might be related with the down-regulation of adenosine A1 receptor expression.

Key words

Caffeine citrate / Brain damage / Myelin basic protein / Adenosine receptor / Neonatal rats

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XU Fa-Lin, CHENG Hui-Qing, WANG Cai-Hong, ZHANG Yan-Hua, GUO Jia-Jia. Effects of caffeine citrate on myelin basic protein in neonatal rats with hypoxicischemic brain damage[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(9): 984-988 https://doi.org/10.7499/j.issn.1008-8830.2015.09.020

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