Establishment and validation of a neonatal pig model of hemolytic jaundice

LI Yong-Fu; MA Yue-Lan; NIE Ling; CHEN Shuan; JIN Mei-Fang; WANG San-Lan

doi:10.7499/j.issn.1008-8830.2016.05.011

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Chinese Journal of Contemporary Pediatrics ›› 2016, Vol. 18 ›› Issue (5) : 431-434. DOI: 10.7499/j.issn.1008-8830.2016.05.011

EXPERIMENTAL RESEARCH

Establishment and validation of a neonatal pig model of hemolytic jaundice

LI Yong-Fu¹, MA Yue-Lan¹, NIE Ling¹, CHEN Shuan¹, JIN Mei-Fang², WANG San-Lan¹

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Abstract

Objective To establish a neonatal pig model of hemolytic jaundice. Methods Twelve sevenday-old purebred Yorkshire pigs were randomly divided into an experimental group and a control group (n=6 each). Immunization of New Zealand white rabbits was used to prepare rabbit anti-porcine red blood cell antibodies, and rabbit anti-porcine red blood cell serum was separated. The neonatal pigs in the experimental group were given an intravenous injection of rabbit anti-porcine red blood cell serum (5 mL), and those in the control group were given an intravenous injection of normal saline (5 mL). Venous blood samples were collected every 6 hours for routine blood test and liver function evaluation. Results The experimental group had a significantly higher serum bilirubin level than the control group at 18 hours after the injection of rabbit anti-porcine red blood cell serum (64±30 μmol/L vs 20±4 μmol/L; P<0.05). In the experimental group, the serum bilirubin level reached the peak at 48 hours (275±31 μmol/L), and decreased significantly at 96 hours after the injection (95±17 μmol/L), but all significantly higher than that in the control group (P<0.05). At 18 hours after the injection, the experimental group had a significantly lower red blood cell (RBC) count than the control group [(4.58±0.32)×10¹²/L vs (5.09±0.44)×10¹²/L; P<0.05]; at 24 hours, the experimental group showed further reductions in RBC count and hemoglobin level and had significantly lower RBC count and hemoglobin level than the control group [RBC: (4.21±0.24)×10¹²/L vs (5.11±0.39)×10¹²/L, P<0.05; hemoglobin: 87±3 g vs 97±6 g, P<0.05]. The differences in RBC count and hemoglobin level between the two groups were largest at 36-48 hours. Conclusions The neonatal pig model of hemolytic jaundice simulates the pathological process of human hemolytic jaundice well and provides good biological and material bases for further investigation of neonatal hemolysis.

Key words

Neonatal hemolysis / Hyperbilirubinemia / Animal model / Pigs

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LI Yong-Fu, MA Yue-Lan, NIE Ling, CHEN Shuan, JIN Mei-Fang, WANG San-Lan. Establishment and validation of a neonatal pig model of hemolytic jaundice[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(5): 431-434 https://doi.org/10.7499/j.issn.1008-8830.2016.05.011

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