CJCP
  中文版
  English Version
 
CJCP  2016, Vol. 18 Issue (5): 446-454    DOI: 10.7499/j.issn.1008-8830.2016.05.014
EXPERIMENTAL RESEARCH Current Issue| Next Issue| Archive| Adv Search  | 
Tranilast inhibits myocardial fibrosis in mice with viral myocarditis
WEN Chun, XIE Gui, ZENG Ping, HUANG Lin-Feng, CHEN Chun-Yuan
Third Xiangya Hospital, Central South University, Changsha 410013, China
Download: PDF (4807 KB)   HTML(88)
Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract  Objective To investigate the effect of tranilast on myocardial fibrosis in mice with viral myocarditis (VMC). Methods Male balb/c mice (n=72) were randomly divided into control, VMC and tranilast groups (n=24 each). In the VMC and tranilast groups, the mice were infected with Coxsackie virus B3 (CVB3) to prepare VMC model, while the control group was treated with Eagle's medium. After modeling, the tranilast group was administrated with tranilast [200 mg/(kg.d)] until the day before sampling. On days 7, 14 and 28 after CVB3 or Eagle's medium infection, heart specimens (n=8) were taken and examined after Toluidine blue staining and Nissl staining for counts of mast cells (MC), hematoxylin-eosin staining for myocardial pathological changes, and Masson staining for myocardial fibrosis. The expression of CTGF and type I collagen (Col I) in the myocardial tissue was measured by RT-PCR and Western blot. The correlations of CTGF mRNA expression with MC counts and Col I expression were analyzed. Results The myocardial pathological changes and collagen volume fraction in the VMC group were significantly higher than in the control group at all three time points (PPPr=0.439, P=0.049) in the VMC group. There were positive correlations between the mRNA expression of Col I and CTGF on the 7th day and 14th day (r=0.646, P=0.007) and the 28th day (r=0.326, P=0.031). Conclusions Tranilast may inhibit the aggregation of MC and down-regulate the expression of CTGF, relieving myocardial fibrosis of mice with VMC.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WEN Chun
XIE Gui
ZENG Ping
HUANG Lin-Feng
CHEN Chun-Yuan
Key wordsTranilast      Viral myocarditis      Myocardial fibrosis      Connective tissue growth factor      Type I collagen      Mice     
Received: 06 February 2016     
Cite this article:   
WEN Chun,XIE Gui,ZENG Ping et al. Tranilast inhibits myocardial fibrosis in mice with viral myocarditis[J]. CJCP, 2016, 18(5): 446-454.
WEN Chun,XIE Gui,ZENG Ping et al. Tranilast inhibits myocardial fibrosis in mice with viral myocarditis[J]. CJCP, 2016, 18(5): 446-454.
URL:  
http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2016.05.014     OR     http://www.zgddek.com/EN/Y2016/V18/I5/446
Copyright © 2004-2005 Chinese Journal Of Contemporary Pediatrics All rights reserved.
Addr: 87 Xiangya Road, Changsha, Hunan, 410008, PRC
Tel: 0731-84327402 Fax: 0731-84327922 E-mail: cjcp1999@csu.edu.cn