Abstract Objective To study the role of the PI3K/AKT signaling pathway in the diallyl disulfide (DADS)-induced apoptosis of K562 cells. Methods K562 cells in the logarithmic growth phase were treated with 10, 20, 40, or 80 mg/L DADS for 48 hours, then fixed and stained with acridine orange/ethidium bromide (AO/EB), and examined for cellular morphological changes under an inverted microscope. Annexin V-FITC/PI staining was used for determining the apoptotic rates, and Western blot for measuring the expression of AKT, p-AKT, and Caspase-3. Two control groups, blank and solvent, were used as references. Results K562 cells treated with DADS for 48 hours exhibited the characteristic morphological features of apoptosis including cell shrinkage, irregular cell shape, and membrane blebbing. AO/EB staining results demonstrated that the number of apoptotic cells with cell shrinkage, pyknotic or bead-like nuclei, chromatin condensation, and orange staining increased with the increasing DADS concentration, and 40 mg/L DADS had the most significant effect. The apoptotic rates of cells treated with 10, 20, 40, and 80 mg/L DADS were all significantly higher than those in the control groups (P P Conclusions DADS induces the apoptosis of K562 cells, probably through inhibiting the protein expression in the PI3K/AKT signaling pathway.
XIAO Liang,YIN Xiao-Cheng,CAO Qiang-Qiang. The role of the PI3K/AKT signaling pathway in DADS-induced apoptosis of K562 cells[J]. CJCP, 2016, 18(10): 1050-1054.
XIAO Liang,YIN Xiao-Cheng,CAO Qiang-Qiang. The role of the PI3K/AKT signaling pathway in DADS-induced apoptosis of K562 cells[J]. CJCP, 2016, 18(10): 1050-1054.
Yi L, Su Q. Molecular mechanisms for anticancer effect of diallyl disulfide[J]. Food Chem Toxicol, 2013, 57: 362-370.
[2]
Miao RZ, Liu LQ, Chen L, et al. Activity of heme oxygenase-1 affects expression levels of hypoxia inducible factor-1 gene in vitro[J]. Chin Med J (Engl), 2012, 125(7): 1310-1315.
[3]
Hollander MC, Blumenthal GM, Dennis PA. PTEN loss in the continuum of common cancers, rare syndromes and mouse models[J]. Nat Rev Cancer, 2011, 11(4): 289-301.
[4]
Pandurangan AK. Potential targets for prevention of colorectal cancer: a focus on PI3K/Akt/mTOR and Wnt pathways[J]. Asian Pac J Cancer Prev, 2013, 14(4): 2201-2205.
Yi L, Su Q. Molecular mechanisms for the anti-cancer effects of diallyl disulfide[J]. Food Chem Toxicol, 2013, 57: 362-370.
[7]
Ji C, Ren F, Ma H, et al. The roles of p38MAPK and caspase-3 in DADS-induced apoptosis in human HepG2 cells[J]. J Exp Clin Cancer Res, 2010, 29: 50.
[8]
Altonsy MO, Andrews SC. Diallyl disulphide, a beneficial component of garlic oil, causes a redistribution of cell-cycle growth phases, induces apoptosis, and enhances butyrateinduced apoptosis in colorectal adenocarcinoma cells(HT-29)[J]. Nutr Cancer, 2011, 63(7): 1104-1113.
[9]
Riggio M, Polo ML, Blaustein M, et al. PI3K/AKT pathway regulates phosphorylation of steroid receptors, hormone independence and tumor differentiation in breast cancer[J]. Carcinogenesis, 2012, 33(3): 509-518.
[10]
Carden CP, Stewart A, Thavasu P, et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer[J]. Mol Cancer Ther, 2012, 11(7): 1609-1617.
[11]
Papadimitrakopoulou V. Development of PI3K/AKT/mTOR pathway inhibitors and their application in personalized therapy for non-small-cell lung cance[J]. J Thorac Oncol, 2012, 7(8): 1315-1326.
