Abstract Objective To study the expression of plasma miRNA-497 in children with sepsis-induced myocardial injury and its clinical significance.Methods A total of 148 children with sepsis were enrolled. According to the presence or absence of myocardial injury, these children were divided into myocardial injury group (n=58) and non-myocardial injury group (n=90). The two groups were compared in terms of the changes in plasma levels of miRNA-497, cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), N-terminal pro-brain natriuretic peptide (NT-proBNP), procalcitonin (PCT), and C-reactive protein (CRP) and left ventricular ejection fraction (LVEF). The receiver operating characteristic (ROC) curve was plotted to evaluate the value of plasma miRNA-497, cTnI, and CK-MB in the diagnosis of myocardial injury. A Pearson correlation analysis was used to determine the correlation of miRNA-497 with cTnI, CK-MB, NT-proBNP, PCT, CRP, and LVEF.Results Compared with the non-myocardial injury group, the myocardial injury group had significantly higher plasma levels of miRNA-497, cTnI, CK-MB, NT-proBNP, PCT, and CRP (P<0.05). Plasma miRNA-497, cTnI, and CK-MB when measured alone or in combination had an area under the ROC curve of 0.918, 0.931, 0.775, and 0.940 respectively. At the optimal cut-off value of 2.05, miRNA-497 had a sensitivity of 90.4% and a specificity of 91.2%. The correlation analysis showed that there was a good correlation between plasma miRNA-497 and cTnI in children with myocardial injury (r=0.728,P<0.01).Conclusions Plasma miRNA-497 has a similar value as cTnI in the diagnosis of sepsis-induced myocardial injury in children and may be used as a potential marker for early diagnosis of myocardial injury.
WU Zhong-Jing,CHEN Ya-Feng,WANG Hai-Dao et al. Expression of plasma miRNA-497 in children with sepsis-induced myocardial injury and its clinical significance[J]. CJCP, 2018, 20(1): 32-36.
WU Zhong-Jing,CHEN Ya-Feng,WANG Hai-Dao et al. Expression of plasma miRNA-497 in children with sepsis-induced myocardial injury and its clinical significance[J]. CJCP, 2018, 20(1): 32-36.
de Souza DC, Shieh HH, Barreira ER, et al. Epidemiology of sepsis in children admitted to PICUs in South America[J]. Pediatr Crit Care Med, 2016, 17(8):727-734.
[2]
Jayaprakash N, Gajic O, Frank RD, et al. Elevated modified shock index in early sepsis is associated with myocardial dysfunction and mortality[J]. J Crit Care, 2017, 43:30-35.
[3]
Kingsley SMK, Bhat BV. Role of microRNAs in sepsis[J]. Inflamm Res, 2017, 66(7):553-569.
[4]
Li Z, Lu J, Luo Y, et al. High association between human circulating microRNA-497 and acute myocardial infarction[J]. Sci World J, 2014:931845.
Corsten MF, Dennert R, Jochems S, et al. Circulating MicroRNA-208b and MicroRNA-499 reflect myocardial damage in cardiovascular disease[J]. Circ Cardiovasc Genet, 2010, 3(6):499-506.
[7]
Maciejak A, Kiliszek M, Opolski G, et al. miR-22-5p revealed as a potential biomarker involved in the acute phase of myocardial infarction via profiling of circulating microRNAs[J]. Mol Med Rep, 2016, 14(3):2867-2875.
[8]
Boon RA, Dimmeler S. MicroRNAs in myocardial infarction[J]. Nat Rev Cardiol, 2015, 12(3):135-142.
Li C, Fang Z, Jiang T, et al. Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris[J]. BMC Med Genomics, 2013, 6:16.
[11]
D'Alessandra Y, Devanna P, Limana F, et al. Circulating microRNAs are new and sensitive biomarkers of myocardial infarction[J]. Eur Heart J, 2010, 31(22):2765-2773.
[12]
Deddens JC, Colijn JM, Oerlemans MI, et al. Circulating microRNAs as novel biomarkers for the early diagnosis of acute coronary syndrome[J]. J Cardiovasc Transl Res, 2013, 6(6):884-898.
[13]
Fan J, Ma J, Xia N, et al. Clinical value of combined detection of CK-MB, MYO, cTnI and plasma NT-proBNP in diagnosis of acute myocardial infarction[J]. Clin Lab, 2017, 63(3):427-433.
[14]
Li HX, Liu ZM, Zhao SJ, et al. Measuring both procalcitonin and C-reactive protein for a diagnosis of sepsis in critically ill patients[J]. J Int Med Res, 2014, 42(4):1050-1059.
[15]
Yang W, Shao J, Bai X, et al. Expression of plasma microRNA-1/21/208a/499 in myocardial ischemic reperfusion injury[J]. Cardiology, 2015, 130(4):237-241.
