Abstract Objective To investigate the association between intestinal bifidobacteria and allergic diseases in infants by comparing the composition of intestinal bifidobacteria between healthy infants and infants with allergic diseases. Methods A total of 48 infants were enrolled, and fecal samples were collected on days 0, 2, 7, and 15 and at months 1, 6, and 12 after birth. Among these infants, 22 who experienced allergic diseases before the age of 1 year were enrolled as allergic group and 26 healthy infants were enrolled as healthy group. Quantitative real-time PCR was used for the qualitative and quantitative analyses of Bifidobacterium and 8 species of bifidobacteria in fecal samples. Results There was a difference in the composition of intestinal bifidobacteria between the two groups within 1 month after birth:the healthy group showed a reduction in bifidobacteria on day 2, while this feature was not observed in the allergic group. Compared with the healthy group, the allergic group had a significantly lower detection count of Bifidobacterium at month 1 (P < 0.05) and a significantly lower detection rate of B.breve on day 15 (P < 0.05), with delayed colonization of B.infantis. Conclusions Intestinal bifidobacteria and their composition within 1 month after birth may be associated with the development of allergic diseases, and this period of time may be a critical period for the prevention and treatment of allergic diseases in infants.
WANG Mao-Lin,SHEN Xi,GE Lin et al. A preliminary study of the difference in composition of intestinal bifidobacteria between healthy infants and infants with allergic diseases[J]. CJCP, 2018, 20(9): 746-752.
WANG Mao-Lin,SHEN Xi,GE Lin et al. A preliminary study of the difference in composition of intestinal bifidobacteria between healthy infants and infants with allergic diseases[J]. CJCP, 2018, 20(9): 746-752.
Warner JO, Kaliner MA, Crisci CD, et al. Allergy practice worldwide:a report by the World Allergy Organization Specialty and Training Council[J]. Int Arch Allergy Immunol, 2006, 139(2):166-174.
[2]
Strachan DP. Hay fever, hygiene, and household size[J]. BMJ, 1989, 299(6710):1259-1260.
[3]
Bokulich NA, Chung J, Battaglia T, et al. Antibiotics, birth mode, and diet shape microbiome maturation during early life[J]. Sci Transl Med, 2016, 8(343):343ra82.
[4]
Ling Z, Li Z, Liu X, et al. Altered fecal microbiota composition associated with food allergy in infants[J]. Appl Environ Microbiol, 2014, 80(8):2546-2554.
[5]
Stokholm J, Blaser MJ, Thorsen J, et al. Maturation of the gut microbiome and risk of asthma in childhood[J]. Nat Commun, 2018, 9(1):141.
[6]
Rautava S, Luoto R, Salminen S, et al. Microbial contact during pregnancy, intestinal colonization and human disease[J]. Nat Rev Gastroenterol Hepatol, 2012, 9(10):565-576.
[7]
Fanaro S, Chierici R, Guerrini P, et al. Intestinal microflora in early infancy:composition and development[J]. Acta Paediatr Suppl, 2003, 91(441):48-55.
[8]
Bisgaard H, Li N, Bonnelykke K, et al. Reduced diversity of the intestinal microbiota during infancy is associated with increased risk of allergic disease at school age[J]. J Allergy Clin Immunol, 2011, 128(3):646-652.e1-5.
[9]
Nylund L, Satokari R, Nikkilä J, et al. Microarray analysis reveals marked intestinal microbiota aberrancy in infants having eczema compared to healthy children in at-risk for atopic disease[J]. BMC Microbiol, 2013, 13:12.
Sjögren YM, Tomicic S, Lundberg A, et al. Influence of early gut microbiota on the maturation of childhood mucosal and systemic immune responses[J]. Clin Exp Allergy, 2009, 39(12):1842-1851.
[14]
Nagpal R, Kurakawa T, Tsuji H, et al. Evolution of gut Bifidobacterium population in healthy Japanese infants over the first three years of life:a quantitative assessment[J]. Sci Rep, 2017, 7(1):10097.
[15]
Martin R, Makino H, Cetinyurek Yavuz A, et al. Early-life events, including mode of delivery and type of feeding, siblings and gender, shape the developing gut microbiota[J]. PLoS One, 2016, 11(6):e0158498.
Zuccotti G, Meneghin F, Aceti A, et al. Probiotics for prevention of atopic diseases in infants:systematic review and meta-analysis[J]. Allergy, 2015, 70(11):1356-1371.
[18]
He F, Ouwehand AC, Isolauri E, et al. Comparison of mucosal adhesion and species identification of bifidobacteria isolated from healthy and allergic infants[J]. FEMS Immunol Med Microbiol, 2001, 30(1):43-47.
[19]
Young SL, Simon MA, Baird MA, et al. Bifidobacterial species differentially affect expression of cell surface markers and cytokines of dendritic cells harvested from cord blood[J]. Clin Diagn Lab Immunol, 2004, 11(4):686-690.
[20]
Akay HK, Bahar Tokman H, Hatipoglu N, et al. The relationship between bifidobacteria and allergic asthma and/or allergic dermatitis:a prospective study of 0-3 years-old children in Turkey[J]. Anaerobe, 2014, 28:98-103.
ZHANG Ji-Yong, ZHOU Shao-Ming, WANG Shao-Hua, SUI Feng-Xuan, GAO Wu-Hong, LIU Qing, CAI Hua-Bo, JIANG Hong-Ying, LI Wei-Yan, WANG Li-Ting, LI Li, ZHAO Wei, YING Jing, WU Qian-Zhen, WENG Bi-Xia, ZENG Yong-Mei. Risk factors for cow's milk protein allergy in infants: a multicenter survey[J]. CJCP, 2020, 22(1): 42-46.
