Effect of low-concentration paclitaxel on collagen deposition outside rat pulmonary artery smooth muscle cells and related mechanism

LUO Yan; XIE Liang; LIU Han-Min; LIU Bin

doi:10.7499/j.issn.1008-8830.2019.09.016

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Chinese Journal of Contemporary Pediatrics ›› 2019, Vol. 21 ›› Issue (9) : 924-929. DOI: 10.7499/j.issn.1008-8830.2019.09.016

EXPERIMENTAL RESEARCH

Effect of low-concentration paclitaxel on collagen deposition outside rat pulmonary artery smooth muscle cells and related mechanism

LUO Yan¹, XIE Liang², LIU Han-Min², LIU Bin¹

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Abstract

Objective To study the effect of low-concentration paclitaxel (PTX) on transforming growth factor-β1 (TGF-β1)-induced collagen deposition outside rat pulmonary artery smooth muscle cells (PASMCs) and related mechanism. Methods Primary rat PASMCs were divided into a blank control group (n=3), a model group (n=3), and a drug intervention group (n=3). No treatment was given for the blank control group. The model group was treated with TGF-β1 with a final concentration of 10 ng/mL. The drug intervention group was treated with PTX with a final concentration of 100 nmol/L in addition to the treatment in the model group. MTT colorimetry was used to measure cell proliferation. Quantitative real-time PCR was used to measure the relative mRNA expression of collagen type I (COL-I) and collagen type Ⅲ (COL-Ⅲ). ELISA was used to measure the OD value of COL-I and COL-Ⅲ proteins. Western blot was used to measure the relative protein expression of COL-I, COL-Ⅲ, and the key proteins of the TGF-β1/Smad3 signaling pathway (Smad3 and p-Smad3). Results Compared with the blank control group, the model group had significant increases in proliferation ability, relative mRNA and protein expression of COL-I and COL-Ⅲ, and relative protein expression of p-Smad3 (P < 0.05). Compared with the model group, the drug intervention group had significant reductions in the above indicators, but which were still higher than those in the blank control group (P < 0.05). There was no significant difference in the relative protein expression of Smad3 among the three groups (P > 0.05). Conclusions Low-concentration PTX exerts a marked inhibitory effect on TGF-β1-induced collagen deposition outside PASMCs, possibly by regulating the phosphorylation of Smad3 protein.

Key words

Collagen deposition / Pulmonary artery smooth muscle cell / Paclitaxel / Transforming growth factor-β1 / Rats

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LUO Yan, XIE Liang, LIU Han-Min, LIU Bin. Effect of low-concentration paclitaxel on collagen deposition outside rat pulmonary artery smooth muscle cells and related mechanism[J]. Chinese Journal of Contemporary Pediatrics. 2019, 21(9): 924-929 https://doi.org/10.7499/j.issn.1008-8830.2019.09.016

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