Association of interleukin-23 receptor and interleukin-17 single nucleotide polymorphisms with necrotizing enterocolitis in Chinese Han preterm infants
ZHANG Yun, LI Ke
Department of Neonatology, Women's and Children's Hospital of Qinghai Province, Xining 810000, China
Abstract Objective To study the association of single nucleotide polymorphisms (SNPs) of interleukin-23 receptor (IL-23R) rs10889677, interleukin-17A (IL-17A) rs227591, and interleukin-17F (IL-17F) rs763780 with necrotizing enterocolitis (NEC) in Chinese Han preterm infants. Methods A total of 100 Chinese Han preterm infants with NEC who were admitted to the neonatal intensive care unit from January 2017 to January 2019 were prospectively enrolled. Of the 100 preterm infants, 63 had stage II NEC and 37 had stage III NEC. A total of 100 preterm infants, matched for age and sex, were selected as the control group. PCR and Sanger sequencing were used to determine the SNPs of rs10889677, rs2275913, and rs763780. An unconditional logistic regression analysis was used to investigate the association of SNPs with NEC susceptibility and severity. Results The genotype and allele frequencies of rs10889677 and rs2275913 had no influence on the development of NEC (P > 0.05). The genotype of rs763780 had no influence on the development of NEC (P > 0.05), but the risk of NEC in the infants carrying C allele was 1.652 times that in those carrying T allele (95%CI:1.052-2.695, P CI:1.045-3.201, P CI:1.052-6.330, P CI:1.034-4.093, P Conclusions The SNPs of IL-23R rs10889677 and IL-17A rs2275913 are not associated with the susceptibility to NEC in Chinese Han preterm infants, while TC+CC genotype and C allele of IL-17F rs763780 are associated with the susceptibility to NEC and the severity of NEC.
ZHANG Yun,LI Ke. Association of interleukin-23 receptor and interleukin-17 single nucleotide polymorphisms with necrotizing enterocolitis in Chinese Han preterm infants[J]. CJCP, 2020, 22(2): 141-145.
ZHANG Yun,LI Ke. Association of interleukin-23 receptor and interleukin-17 single nucleotide polymorphisms with necrotizing enterocolitis in Chinese Han preterm infants[J]. CJCP, 2020, 22(2): 141-145.
Eaton S, Rees CM, Hall NJ. Current research on the epidemiology, pathogenesis, and management of necrotizing enterocolitis[J]. Neonatology, 2017, 111(4):423-430.
[3]
Treszl A, Heninger E, Kalman A, et al. Lower prevalence of IL-4 receptor alpha-chain gene G variant in very-low-birth-weight infants with necrotizing enterocolitis[J]. J Pediatr Surg, 2003, 38(9):1374-1378.
[4]
Cuna A, Sampath V. Genetic alterations in necrotizing enterocolitis[J]. Semin Perinatol, 2017, 41(1):61-69.
Babaie F, Hasankhani M, Mohammadi H, et al. The role of gut microbiota and IL-23/IL-17 pathway in ankylosing spondylitis immunopathogenesis:new insights and updates[J]. Immunol Lett, 2018, 196:52-62.
[7]
Kim SW, Kim ES, Moon CM, et al. Genetic polymorphisms of IL-23R and IL-17A and novel insights into their associations with inflammatory bowel disease[J]. Gut, 2011, 60(11):1527-1536.
[8]
Bell MJ, Ternberg JL, Feigin RD, et al. Neonatal necrotizing enterocolitis. Therapeutic decisions based upon clinical staging[J]. Ann Surg, 1978, 187(1):1-7.
[9]
Ma F, Li S, Gao X, et al. Interleukin-6-mediated CCR9+ interleukin-17-producing regulatory T cells polarization increases the severity of necrotizing enterocolitis[J]. EBioMedicine, 2019, 44:71-85.
[10]
Lawrence SM, Ruoss JL, Wynn JL. IL-17 in neonatal health and disease[J]. Am J Reprod Immunol, 2018, 79(5):e12800.
McGovern D, Powrie F. The IL23 axis plays a key role in the pathogenesis of IBD[J]. Gut, 2007, 56(10):1333-1336.
[13]
Walsh MC, Kliegman RM. Necrotizing enterocolitis:treatment based on staging criteria[J]. Pediatr Clin North Am, 1986, 33(1):179-201.
[14]
Luo Y, Luo J, Peng H. Associations between genetic polymorphisms in the VEGFA, ACE, and SOD2 genes and susceptibility to diabetic nephropathy in the Han Chinese[J]. Genet Test Mol Biomarkers, 2019, 23(9):644-651.
Hammad A, Mosaad YM, Hammad EM, et al. Interleukin-17A rs2275913, Interleukin-17F rs763780 and rs2397084 gene polymorphisms as possible risk factors in Juvenile lupus and lupus related nephritis[J]. Autoimmunity, 2016, 49(1):31-40.
[17]
Jiang L, Zhou X, Xiong Y, et al. Association between interleukin-17A/F single nucleotide polymorphisms and susceptibility to osteoarthritis in a Chinese population[J]. Medicine (Baltimore), 2019, 98(12):e14944.
[18]
Eskandari-Nasab E, Moghadampour M, Tahmasebi A. Meta-analysis of risk association between interleukin-17A and F gene polymorphisms and inflammatory diseases[J]. J Interferon Cytokine Res, 2017, 37(4):165-174.
[19]
Egan CE, Sodhi CP, Good M, et al. Toll-like receptor 4-mediated lymphocyte influx induces neonatal necrotizing enterocolitis[J]. J Clin Invest, 2016, 126(2):495-508.
[20]
Gao X, Ma F, Hao H, et al. Association of VEGFA polymorphisms with necrotizing enterocolitis in Chinese Han population[J]. Pediatr Neonatol, 2019, 60(2):129-134.