Protective effect of transplantation of human oligodendrocyte precursor cells in a rat model of white matter injury
ZHONG Xin1, LUAN Zuo2, ZANG Jing2, GUAN Qian2, YANG Yin-Xiang2, WANG Qian2, SHI Yuan1
Department of Neonatology, Children's Hospital of Chongqing Medical University/National Clinical Research Center for Child Health and Disorders/Ministry of Education Key Laboratory of Child Development and Disorders/Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China
Abstract Objective To study the effect of human oligodendrocyte precursor cell (hOPC) transplantation in the treatment of white matter injury (WMI). Methods Neonatal rats were randomly divided into a sham-operation group, a model group, and a transplantation group (n=10 each). At the age of 3 days, the rats in the model group and the transplantation group were treated with right common carotid artery ligation, followed by hypoxia for 2 hours, to prepare a rat model of WMI. hOPCs were isolated from a spontaneously aborted human fetal brain at week 11 of gestation, and then hOPCs were cultured and transplanted into the rats with WMI. At 3 months after transplantation, the water maze test was performed to evaluate neurological function, and an electron microscope was used to observe myelin sheath thickness and proliferation. Results The place navigation test using the Morris water maze showed that the model group had a significantly longer escape latency than the sham-operation group, and compared with the model group, the transplantation group had a significant reduction in escape latency (P < 0.05). To a certain degree, hOPC transplantation alleviated cognitive impairment in rats with WMI at the age of 90 days. The electron microscope images showed that hOPC transplantation promoted remyelination in the brain of WMI rats. Compared with the sham-operation group, the model group had a significant increase in the g-ratio (total axon diameter/total fiber diameter). Compared with the model group, the transplantation group had a significant reduction in the g-ratio (P < 0.05). Conclusions Intrathecal hOPC transplantation may alleviate neurological injury and promote remyelination in a rat model of WMI.
ZHONG Xin,LUAN Zuo,ZANG Jing et al. Protective effect of transplantation of human oligodendrocyte precursor cells in a rat model of white matter injury[J]. CJCP, 2021, 23(4): 410-415.
ZHONG Xin,LUAN Zuo,ZANG Jing et al. Protective effect of transplantation of human oligodendrocyte precursor cells in a rat model of white matter injury[J]. CJCP, 2021, 23(4): 410-415.
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