广谱抗生素疗程对极低出生体重儿粪便肠道菌群和短链脂肪酸影响的前瞻性研究

doi:10.7499/j.issn.1008-8830.2107103

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Abstract Objective To study the effect of the course of treatment with broad-spectrum antibiotics on intestinal flora and short-chain fatty acids (SCFAs) in feces of very low birth weight (VLBW) infants. Methods A total of 29 VLBW infants who were admitted to the Neonatal Diagnosis and Treatment Center of Children's Hospital Affiliated to Chongqing Medical University from June to December 2020 were enrolled as subjects for this prospective study. According to the course of treatment with broad-spectrum antibiotics, they were divided into two groups: ≤7 days (n=9) and >7 days (n=20). Fecal samples were collected on days 14 and 28 of hospitalization, and 16S rDNA high-throughput sequencing and gas chromatography-mass spectrometry were used to analyze the flora and SCFAs in fecal samples. Results There was a significant reduction in Chao index of the intestinal flora in the ≤7 days group and the >7 days group from week 2 to week 4 (P<0.05). In the ≤7 days group, there were significant increases in the proportions of Firmicutes and Clostridium_sensu_stricto_1 and a significant reduction in the proportion of Proteobacteria from week 2 to week 4 (P<0.05). At week 4, compared with the ≤7 days group, the >7 days group had significant reductions in the proportions of Firmicutes and Clostridium_sensu_stricto_1 and a significant increase in the proportion of Proteobacteria (P<0.05), as well as significant reductions in the content of isobutyric acid and valeric acid (P<0.05). Conclusions The course of treatment with broad-spectrum antibiotics can affect the abundance, colonization, and evolution of intestinal flora and the content of their metabolites SCFAs in VLBW infants. The indication and treatment course for broad-spectrum antibiotics should be strictly controlled in clinical practice.

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	SUN Qian
	WANG Zheng-Li
	LIU Xiao-Chen
	JI Yan-Chun
	HE Yu
	AI Qing
	LI Lu-Quan

Key words： Broad-spectrum antibiotics Intestinal flora Short-chain fatty acid Very low birth weight infant

Received: 21 July 2021

Cite this article:

SUN Qian,WANG Zheng-Li,LIU Xiao-Chen et al. Effect of the course of treatment with broad-spectrum antibiotics on intestinal flora and short-chain fatty acids in feces of very low birth weight infants: a prospective study[J]. CJCP, 2021, 23(10): 1008-1014.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2107103 OR http://www.zgddek.com/EN/Y2021/V23/I10/1008

	1 李瑶, 黄金莉, 黄娟, 等. 肠道菌群与肠道屏障互作在炎症性肠病中的作用研究进展[J]. 胃肠病学和肝病学杂志, 2021, 30(1): 10-15. DOI: 10.3969/j.issn.1006-5709.2021.01.003.
	Chen YW, Zhou JH, Wang L. Role and mechanism of gut microbiota in human disease[J]. Front Cell Infect Microbiol, 2021, 11: 625913. PMID: 33816335. PMCID: PMC8010197. DOI: 10.3389/fcimb.2021.625913.
	Costello EK, Carlisle EM, Bik EM, et al. Microbiome assembly across multiple body sites in low-birthweight infants[J]. mBio, 2013, 4(6): e00782-13. PMID: 24169577. PMCID: PMC3809564. DOI: 10.1128/mBio.00782-13.
	贾琼, 童笑梅. 早产儿菌群特征及与疾病关系的研究进展[J]. 中国当代儿科杂志, 2020, 22(11): 1240-1244. PMID: 33172562. PMCID: PMC7666391. DOI: 10.7499/j.issn.1008-8830.2005131.
	Ting JY, Roberts A, Sherlock R, et al. Duration of initial empirical antibiotic therapy and outcomes in very low birth weight infants[J]. Pediatrics, 2019, 143(3): e20182286. PMID: 30819968. DOI: 10.1542/peds.2018-2286.
	Chen YX, Xie YN, Zhong RQ, et al. Effects of xylo-oligosaccharides on growth and gut microbiota as potential replacements for antibiotic in weaning piglets[J]. Front Microbiol, 2021, 12: 641172. PMID: 33717037. PMCID: PMC7947891. DOI: 10.3389/fmicb.2021.641172.
	Wandro S, Osborne S, Enriquez C, et al. The microbiome and metabolome of preterm infant stool are personalized and not driven by health outcomes, including necrotizing enterocolitis and late-onset sepsis[J]. mSphere, 2018, 3(3): e00104-18. PMID: 29875143. PMCID: PMC5990886. DOI: 10.1128/mSphere.00104-18.
	Liu WX, Luo XL, Tang J, et al. A bridge for short-chain fatty acids to affect inflammatory bowel disease, type 1 diabetes, and non-alcoholic fatty liver disease positively: by changing gut barrier[J]. Eur J Nutr, 2021, 60(5): 2317-2330. PMID: 33180143. DOI: 10.1007/s00394-020-02431-w.
	Zhu DP, Xiao S, Yu JL, et al. Effects of one-week empirical antibiotic therapy on the early development of gut microbiota and metabolites in preterm infants[J]. Sci Rep, 2017, 7(1): 8025. PMID: 28808302. PMCID: PMC5556106. DOI: 10.1038/s41598-017-08530-9.
	Arboleya S, Sánchez B, Solís G, et al. Impact of prematurity and perinatal antibiotics on the developing intestinal microbiota: a functional inference study[J]. Int J Mol Sci, 2016, 17(5): 649. PMID: 27136545. PMCID: PMC4881475. DOI: 10.3390/ijms17050649.
	Risely A, Gillingham MAF, Béchet A, et al. Phylogeny- and abundance-based metrics allow for the consistent comparison of core gut microbiome diversity indices across host species[J]. Front Microbiol, 2021, 12: 659918. PMID: 34046023. PMCID: PMC8144293. DOI: 10.3389/fmicb.2021.659918.
	Su LL, Mao CQ, Wang XC, et al. The anti-colitis effect of schisandra chinensis polysaccharide is associated with the regulation of the composition and metabolism of gut microbiota[J]. Front Cell Infect Microbiol, 2020, 10: 519479. PMID: 33194780. PMCID: PMC7609416. DOI: 10.3389/fcimb.2020.519479.
	13 Tsukuda N, Yahagi K, Hara T, et al. Key bacterial taxa and metabolic pathways affecting gut short-chain fatty acid profiles in early life[J]. ISME J, 2021. PMID: 33723382. DOI: 10.1038/s41396-021-00937-7. Epub ahead of print.
	Greenwood C, Morrow AL, Lagomarcino AJ, et al. Early empiric antibiotic use in preterm infants is associated with lower bacterial diversity and higher relative abundance of Enterobacter[J]. J Pediatr, 2014, 165(1): 23-29. PMID: 24529620. PMCID: PMC4074569. DOI: 10.1016/j.jpeds.2014.01.010.
	Zhu XH, Fu B, Dong MY, et al. Effects of long-term antibiotic treatment on mice urinary aromatic amino acid profiles[J]. Biosci Rep, 2021, 41(1): BSR20203498. PMID: 33269386. PMCID: PMC7786327. DOI: 10.1042/BSR20203498.
	Lu SL, Huang QM, Wei BM, et al. Effects of β-lactam antibiotics on gut microbiota colonization and metabolites in late preterm infants[J]. Curr Microbiol, 2020, 77(12): 3888-3896. PMID: 32970172. DOI: 10.1007/s00284-020-02198-7.
	17 赵怀宝, 任玉龙. 短链脂肪酸在动物体内的生理特点和功能[J]. 饲料研究, 2016(3): 29-32. DOI: 10.13557/j.cnki.issn1002-2813.2016.03.007.
	Arrieta MC, Stiemsma LT, Amenyogbe N, et al. The intestinal microbiome in early life: health and disease[J]. Front Immunol, 2014, 5: 427. PMID: 25250028. PMCID: PMC4155789. DOI: 10.3389/fimmu.2014.00427.
	Liu B, Popp D, Müller N, et al. Three novel Clostridia isolates produce n-Caproate and iso-Butyrate from lactate: comparative genomics of chain-elongating bacteria[J]. Microorganisms, 2020, 8(12): 1970. PMID: 33322390. PMCID: PMC7764203. DOI: 10.3390/microorganisms8121970.
	Heimann E, Nyman M, P?lbrink AK, et al. Branched short-chain fatty acids modulate glucose and lipid metabolism in primary adipocytes[J]. Adipocyte, 2016, 5(4): 359-368. PMID: 27994949. PMCID: PMC5160390. DOI: 10.1080/21623945.2016.1252011.
	Jayaraj RL, Beiram R, Azimullah S, et al. Valeric acid protects dopaminergic neurons by suppressing oxidative stress, neuroinflammation and modulating autophagy pathways[J]. Int J Mol Sci, 2020, 21(20): 7670. PMID: 33081327. PMCID: PMC7589299. DOI: 10.3390/ijms21207670.