儿童急性淋巴细胞白血病中<italic>RAS</italic>基因突变的检测及其临床意义

doi:10.7499/j.issn.1008-8830.2109141

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Abstract Objective To investigate the mutation rate of the RAS gene and its clinical significance in children with acute lymphoblastic leukemia. Methods A retrospective analysis was performed on the medical data of 120 children with newly diagnosed acute lymphoblastic leukemia, who were admitted to the Third Affiliated Hospital of Zhengzhou University from January 2015 to January 2020 and underwent next-generation sequencing. The clinical and molecular features were analyzed. The impact of RAS gene mutation on the overall survival rate was evaluated in these children. Results Among the 120 children, 35 (29.2%) had RAS gene mutation, 30 (25.0%) had KRAS gene mutation, and 5 (4.2%) had both NRAS and KRAS gene mutations. All NRAS mutations and 71% (25/35) of KRAS mutations were located at the 12th and 13th codons. RAS gene mutation was detected in 35 (33.3%) out of 105 children with B-lineage acute lymphoblastic leukemia, but it was not detected in those with acute T lymphocyte leukemia. Of all the children, 11 (9.2%) were lost to follow-up, and among the 109 children followed up, 16 (14.7%) died. The children with RAS gene mutation had a significantly lower 2-year overall survival rate than those without RAS gene mutation (P<0.05). The prognosis of children with RAS gene mutation combined with WT1 overexpression and WBC>50×109/L at diagnosis was worse (P<0.05). Conclusions RAS gene mutation is commonly observed in children with B-lineage acute lymphoblastic leukemia and may have an adverse effect on prognosis.

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	YAO Yan-Ling
	WANG Xi-Ge
	ZHAO Xue-Lian
	LI Shuai-Quan
	ZHOU Yu-Jie
	XU Yi-Zhuo

Key words： Acute lymphoblastic leukemia RAS gene Mutation Prognosis Child

Received: 24 September 2021

Cite this article:

YAO Yan-Ling,WANG Xi-Ge,ZHAO Xue-Lian et al. Detection of RAS gene mutation and its clinical significance in children with acute lymphoblastic leukemia[J]. CJCP, 2022, 24(3): 309-313.

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http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2109141 OR http://www.zgddek.com/EN/Y2022/V24/I3/309

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