Abstract Objective To investigate the genotypes of the pathogenic gene COL4A5 and the characteristics of clinical phenotypes in children with Alport syndrome (AS). Methods A retrospective analysis was performed for the genetic testing results and clinical data of 19 AS children with COL4A5 gene mutations. Results Among the 19 children with AS caused by COL4A5 gene mutations, 1 (5%) carried a new mutation of the COL4A5 gene, i.e., c.3372A>G(p.P1124=) and presented with AS coexisting with IgA vasculitis nephritis; 3 children (16%) had large fragment deletion of the COL4A5 gene, among whom 2 children (case 7 had a new mutation site of loss51-53) had gross hematuria and albuminuria at the onset, and 1 child (case 13 had a new mutation site of loss3-53) only had microscopic hematuria, while the other 15 children (79%) had common clinical phenotypes of AS, among whom 7 carried new mutations of the COL4A5 gene. Among all 19 children, 3 children (16%) who carried COL4A5 gene mutations also had COL4A4 gene mutations, and 1 child (5%) had COL4A3 gene mutations. Among these children with double gene mutations, 2 had gross hematuria and proteinuria at the onset. Conclusions This study expands the genotype and phenotype spectrums of the pathogenic gene COL4A5 for AS. Children with large fragment deletion of the COL4A5 gene or double gene mutations of COL4A5 with COL4A3 or COL4A4 tend to have more serious clinical manifestations.
Savige J, Storey H, Watson E, et al. Consensus statement on standards and guidelines for the molecular diagnostics of Alport syndrome: refining the ACMG criteria[J]. Eur J Hum Genet, 2021, 29(8): 1186-1197. PMID: 33854215. PMCID: PMC8384871. DOI: 10.1038/s41431-021-00858-1.
Matthaiou A, Poulli T, Deltas C. Prevalence of clinical, pathological and molecular features of glomerular basement membrane nephropathy caused by COL4A3 or COL4A4 mutations: a systematic review[J]. Clin Kidney J, 2020, 13(6): 1025-1036. PMID: 33391746. PMCID: PMC7769542. DOI: 10.1093/ckj/sfz176.
Richards S, Aziz N, Bale S, et al. Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology[J]. Genet Med, 2015, 17(5): 405-424. PMID: 25741868. PMCID: PMC4544753. DOI: 10.1038/gim.2015.30.
Choi M, Anistan YM, Eckardt KU, et al. Possible digenic disease in a Caucasian family with COL4A3 and COL4A5 mutations[J]. Nephron, 2019, 141(3): 213-218. PMID: 30661074. DOI: 10.1159/000495764.
Zhang Y, Ding J, Zhang H, et al. Effect of heterozygous pathogenic COL4A3 or COL4A4 variants on patients with X-linked Alport syndrome[J]. Mol Genet Genomic Med, 2019, 7(5): e647. PMID: 30883042. PMCID: PMC6503168. DOI: 10.1002/mgg3.647.
Zhao X, Chen C, Wei Y, et al. Novel mutations of COL4A3, COL4A4, and COL4A5 genes in Chinese patients with Alport syndrome using next generation sequence technique[J]. Mol Genet Genomic Med, 2019, 7(6): e653. PMID: 30968591. PMCID: PMC6565573. DOI: 10.1002/mgg3.653.
Navarro-Cobos MJ, Balaton BP, Brown CJ. Genes that escape from X-chromosome inactivation: potential contributors to Klinefelter syndrome[J]. Am J Med Genet C Semin Med Genet, 2020, 184(2): 226-238. PMID: 32441398. PMCID: PMC7384012. DOI: 10.1002/ajmg.c.31800.
20 Queremel Milani DA, Chauhan PR. Genetics, Mosaicism[M]//StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing, 2023.